Details:

Alternative Title:
PLoS One
Publisher's site:
http://dx.doi.org/10.1371/journal.pone.0201295
Personal Author:
Borkenhagen, Laura K. ; Mallinson, Kerry A. ; Tsao, Rick W. ; Ha, Siaw-Jing ; Lim, Wei-Honn ; ... More +
Borkenhagen, Laura K. ; Mallinson, Kerry A. ; Tsao, Rick W. ; Ha, Siaw-Jing ; Lim, Wei-Honn ; Toh, Teck-Hock ; Anderson, Benjamin D. ; Fieldhouse, Jane K. ; Philo, Sarah E. ; Chong, Kuek-Sen ; Lindsley, William G. ; Ramirez, Alejandro ; Lowe, James F. ; Coleman, Kristen K. ; Gray, Gregory C. Less -
Description:
Background

The large livestock operations and dense human population of Southeast Asia are considered a hot-spot for emerging viruses.

Objectives

To determine if the pathogens adenovirus (ADV), coronavirus (CoV), encephalomyocarditis virus (EMCV), enterovirus (EV), influenza A-D (IAV, IBV, ICV, and IDV), porcine circovirus 2 (PCV2), and porcine rotaviruses A and C (RVA and RVC), are aerosolized at the animal-interface, and if humans working in these environments are carrying these viruses in their nasal airways.

Study

This cross-sectional study took place in Sarawak, Malaysia among 11 pig farms, 2 abattoirs, and 3 animal markets in June and July of 2017. Pig feces, pig oral secretions, bioaerosols, and worker nasal wash samples were collected and analyzed via rPCR and rRT-PCR for respiratory and diarrheal viruses.

Results

In all, 55 pig fecal, 49 pig oral or water, 45 bioaerosol, and 78 worker nasal wash samples were collected across 16 sites. PCV2 was detected in 21 pig fecal, 43 pig oral or water, 3 bioaerosol, and 4 worker nasal wash samples. In addition, one or more bioaerosol or pig samples were positive for EV, IAV, and RVC, and one or more worker samples were positive for ADV, CoV, IBV, and IDV.

Conclusions

This study demonstrates that nucleic acids from a number of targeted viruses were present in pig oral secretions and pig fecal samples, and that several viruses were detected in bioaerosol samples or in the nasal passages of humans with occupational exposure to pigs. These results demonstrate the need for future research in strengthening viral surveillance at the human-animal interface, specifically through expanded bioaerosol sampling efforts and a seroepidemiological study of individuals with exposure to pigs in this region for PCV2 infection.


Subject:
[+]
Animals
Cross-Sectional Studies
Diarrhea
Farmers
Female
Humans
Male
Occupational Exposure
Respiratory Tract Infections
Swine
Swine Diseases
Virus Diseases
Viruses

Source:
PLoS One. 13(7).
Pubmed ID:
30052648
Pubmed Central ID:
PMC6063427
Document Type:
Journal Article
Place as Subject:
Malaysia
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:4603b3c2701e73e8e5ca5b00db305bc7836fa33e23b1708a329557699cf222b4
File Type:

Supporting Files

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