Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens
Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.

Search our Collections & Repository

For very narrow results

When looking for a specific result

Best used for discovery & interchangable words

Recommended to be used in conjunction with other fields

Dates

to

Document Data
Library
People
Clear All
Clear All

For additional assistance using the Custom Query please check out our Help Page

i

Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens

Filetype[PDF-2.56 MB]


Details Supporting Files Datasets You May Also Like

Details:

  • Alternative Title:
    BMC Genomics
  • Personal Author:
  • Description:
    Background

    Cyclospora cayetanensis is an apicomplexan that causes diarrhea in humans. The investigation of foodborne outbreaks of cyclosporiasis has been hampered by a lack of genetic data and poor understanding of pathogen biology. In this study we sequenced the genome of C. cayetanensis and inferred its metabolism and invasion components based on comparative genomic analysis.

    Results

    The genome organization, metabolic capabilities and potential invasion mechanism of C. cayetanensis are very similar to those of Eimeria tenella. Propanoyl-CoA degradation, GPI anchor biosynthesis, and N-glycosylation are some apparent metabolic differences between C. cayetanensis and E. tenella. Unlike Eimeria spp., there are no active LTR-retrotransposons identified in C. cayetanensis. The similar repertoire of host cell invasion-related proteins possessed by all coccidia suggests that C. cayetanensis has an invasion process similar to the one in T. gondii and E. tenella. However, the significant reduction in the number of identifiable rhoptry protein kinases, phosphatases and serine protease inhibitors indicates that monoxenous coccidia, especially C. cayetanensis, have limited capabilities or use a different system to regulate host cell nuclear activities. C. cayetanensis does not possess any cluster of genes encoding the TA4-type SAG surface antigens seen in E. tenella, and may use a different family of surface antigens in initial host cell interactions.

    Conclusions

    Our findings indicate that C. cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens. Amino acid metabolism and post-translation modifications of proteins are some major differences between C. cayetanensis and other apicomplexans. The whole genome sequence data of C. cayetanensis improve our understanding of the biology and evolution of this major foodborne pathogen and facilitate the development of intervention measures and advanced diagnostic tools.

    Electronic supplementary material

    The online version of this article (doi:10.1186/s12864-016-2632-3) contains supplementary material, which is available to authorized users.

  • Subjects:
  • Source:
    BMC Genomics. 17.
  • Pubmed ID:
    27129308
  • Pubmed Central ID:
    PMC4851813
  • Document Type:
    Journal Article
  • Volume:
    17
  • Collection(s):
  • Main Document Checksum:
  • Download URL:
  • File Type:

Datasets

    1. Additional file 8 Table S5 of Comparative genomics reveals Cyclospora cayetanensis possesses coccidialike metabolism and invasion components but unique surface antigens
      Additional file 8: Table S5. of Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens
      Dataset Icon
      Filetype[HTM]
      Personal Author:
      Arrowood, Michael J. ;
      Feng, Yaoyu
      ...
      2016 | NCEZID
      12/14/2016
      Description:
      Members of a major group of paralogs detected in the Cyclospora cayetanensis genome and results of BLASTP against the GenBank protein database. (XLSX ...
      File Type:
      Filetype[HTM]
      File Type:
      Filetype[HTM]
    2. Additional file 6 Table S3 of Comparative genomics reveals Cyclospora cayetanensis possesses coccidialike metabolism and invasion components but unique surface antigens
      Additional file 6: Table S3. of Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens
      Dataset Icon
      Filetype[HTM]
      Personal Author:
      Arrowood, Michael J. ;
      Feng, Yaoyu
      ...
      2016 | NCEZID
      12/14/2016
      Description:
      Comparison of essential cellular metabolic pathways among some common apicomplexan parasites. (XLSX 70 kb)
      File Type:
      Filetype[HTM]
      File Type:
      Filetype[HTM]
    3. Additional file 8 Table S5 of Comparative genomics reveals Cyclospora cayetanensis possesses coccidialike metabolism and invasion components but unique surface antigens
      Additional file 8: Table S5. of Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens
      Dataset Icon
      Filetype[HTM]
      Personal Author:
      Arrowood, Michael J. ;
      Feng, Yaoyu
      ...
      2016 | NCEZID
      12/14/2016
      Description:
      Members of a major group of paralogs detected in the Cyclospora cayetanensis genome and results of BLASTP against the GenBank protein database. (XLSX ...
      File Type:
      Filetype[HTM]
      File Type:
      Filetype[HTM]
    4. Additional file 6 Table S3 of Comparative genomics reveals Cyclospora cayetanensis possesses coccidialike metabolism and invasion components but unique surface antigens
      Additional file 6: Table S3. of Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens
      Dataset Icon
      Filetype[HTM]
      Personal Author:
      Arrowood, Michael J. ;
      Feng, Yaoyu
      ...
      2016 | NCEZID
      12/14/2016
      Description:
      Comparison of essential cellular metabolic pathways among some common apicomplexan parasites. (XLSX 70 kb)
      File Type:
      Filetype[HTM]
      File Type:
      Filetype[HTM]
More +

You May Also Like

Checkout today's featured content at stacks.cdc.gov