TALENs: a widely applicable technology for targeted genome editing

Details

• Alternative Title:
  Nat Rev Mol Cell Biol
• Personal Author:
  Joung, J. Keith ; Sander, Jeffry D.
• Description:
  Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of cell types and organisms. The newly-developed transcription activator-like effector nucleases (TALENs) comprise a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain that can be easily engineered so that TALENs can target essentially any sequence. The capability to quickly and efficiently alter genes using TALENs promises to have profound impacts on biological research and to yield potential therapeutic strategies for genetic diseases.
• Subjects:
  Animals Deoxyribonucleases DNA DNA-Binding Proteins Genome Humans Protein Engineering Protein Structure, Tertiary Transcriptional Activation
• Source:
  Nat Rev Mol Cell Biol. 14(1):49-55.
• Pubmed ID:
  23169466
• Pubmed Central ID:
  PMC3547402
• Document Type:
  Journal Article
• Funding:
  DP1 GM105378/DP/NCCDPHP CDC HHS/United States ; DP1 GM105378/GM/NIGMS NIH HHS/United States ; P50 HG005550/HG/NHGRI NIH HHS/United States ; R01 GM088040/GM/NIGMS NIH HHS/United States ; T32CA009216/CA/NCI NIH HHS/United States
• Volume:
  14
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:a5f6996eb0759ba28d50339aa0e12d0e494e44874de8415ef6ed8cbf3072d579
• Download URL:
  https://stacks.cdc.gov/view/cdc/33504/cdc_33504_DS1.pdf
• File Type:
  [PDF - 1.02 MB ]