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Continuous directed evolution of DNA-binding proteins to improve TALEN specificity
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Aug 10 2015
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Source: Nat Methods. 12(10):939-942.
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Alternative Title:Nat Methods
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Description:Nucleases containing programmable DNA-binding domains can alter the genomes of model organisms and have the potential to become human therapeutics. Here we present DNA-binding phage-assisted continuous evolution (DB-PACE) as a general approach for the laboratory evolution of DNA-binding activity and specificity. We used this system to generate transcription activator-like effectors nucleases (TALENs) with broadly improved DNA cleavage specificity, establishing DB-PACE as a versatile approach for improving the accuracy of genome-editing agents.
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Pubmed ID:26258293
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Pubmed Central ID:PMC4589463
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Volume:12
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Issue:10
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