i
Continuous directed evolution of DNA-binding proteins to improve TALEN specificity
-
Aug 10 2015
Source: Nat Methods. 12(10):939-942.
Details:
-
Alternative Title:Nat Methods
-
Personal Author:
-
Description:Nucleases containing programmable DNA-binding domains can alter the genomes of model organisms and have the potential to become human therapeutics. Here we present DNA-binding phage-assisted continuous evolution (DB-PACE) as a general approach for the laboratory evolution of DNA-binding activity and specificity. We used this system to generate transcription activator-like effectors nucleases (TALENs) with broadly improved DNA cleavage specificity, establishing DB-PACE as a versatile approach for improving the accuracy of genome-editing agents.
-
Subjects:
-
Source:
-
Pubmed ID:26258293
-
Pubmed Central ID:PMC4589463
-
Document Type:
-
Funding:
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
Supporting Files
-
txt pdf bin gif jpeg gif jpeg
More +