Inhalation of Stachybotrys chartarum Fragments Induces Pulmonary Arterial Remodeling

Details

• Alternative Title:
  Am J Respir Cell Mol Biol
• Personal Author:
  Croston, Tara L. ; Lemons, Angela R. ; Barnes, Mark A. ; Goldsmith, William T. ; Orandle, Marlene S. ; Nayak, Ajay P. ; Germolec, Dori R. ; Green, Brett J. ; Beezhold, Donald H.
• Description:
  is a fungal contaminant within the built environment and a respiratory health concern in the United States. The objective of this study was to characterize the mechanisms influencing pulmonary immune responses to repeatedly inhaled |. Groups of B6C3F1/N mice repeatedly inhaled viable trichothecene-producing | conidia (strain A or strain B), heat-inactivated conidia, or high-efficiency particulate absolute-filtered air twice per week for 4 and 13 weeks. Strain A was found to produce higher amounts of respirable fragments than strain B. Lung tissue, serum, and BAL fluid were collected at 24 and 48 hours after final exposure and processed for histology, flow cytometry, and RNA and proteomic analyses. At 4 weeks after exposure, a T-helper cell type 2-mediated response was observed. After 13 weeks, a mixed T-cell response was observed after exposure to strain A compared with a T-helper cell type 2-mediated response after strain B exposure. After exposure, both strains induced pulmonary arterial remodeling at 13 weeks; however, strain A-exposed mice progressed more quickly than strain B-exposed mice. BAL fluid was composed primarily of eosinophils, neutrophils, and macrophages. Both the immune response and the observed pulmonary arterial remodeling were supported by specific cellular, molecular, and proteomic profiles. The immunopathological responses occurred earlier in mice exposed to high fragment-producing strain A. The rather striking induction of pulmonary remodeling by | appears to be related to the presence of fungal fragments during exposure.
• Subjects:
  Administration, Inhalation Animals Bronchoalveolar Lavage Fluid Female Gene Expression Profiling Gene Expression Regulation Lung Diseases, Fungal Mice Microbial Viability MicroRNAs Proteomics Pulmonary Artery Stachybotrys Th1 Cells Th17 Cells Vascular Remodeling

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• Keywords:
  Fungal Exposure Pulmonary Arterial Remodeling Stachybotrys Chartarum Fungal Fragmentation
• Source:
  Am J Respir Cell Mol Biol. 62(5):563-576
• Pubmed ID:
  31671270
• Pubmed Central ID:
  PMC7263392
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  62
• Issue:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:c603b764112ee4c84f51608ba9334fb1fc322c6dcd1e634cbbf3ed5f9cf64c20
• Download URL:
  https://stacks.cdc.gov/view/cdc/90273/cdc_90273_DS1.pdf
• File Type:
  [PDF - 2.63 MB ]