A deep insight into the male and female sialotranscriptome of adult Culex tarsalis mosquitoes

Details

• Alternative Title:
  Insect Biochem Mol Biol
• Personal Author:
  Ribeiro, José M.C. ; Martin-Martin, Ines ; Moreira, Fernando R. ; Bernard, Kristen A. ; Calvo, Eric
• Description:
  Previously, a Sanger-based sialotranscriptome analysis of adult female Culex tarsalis was published based on ∼2000 ESTs. During the elapsed 7.5 years, pyrosequencing has been discontinued and Illumina sequences have increased considerable in size and decreased in price. We here report an Illumina-based sialotranscriptome that allowed finding the missing apyrase from the salivary transcriptome of C. tarsalis, to determine several full-length members of the 34-62 kDa family, when a single EST has been found previously, in addition to identifying many salivary families with lower expression levels that were not detected previously. The use of multiple libraries including salivary glands and carcasses from male and female organisms allowed for an unprecedented insight into the tissue specificity of transcripts, and in this particular case permitting identification of transcripts putatively associated with blood feeding, when exclusive of female salivary glands, or associated with sugar feeding, when transcripts are found upregulated in both male and female glands.
• Subjects:
  Animals Culex Female Gene Expression Profiling Insect Proteins Male Sex Characteristics Sialoglycoproteins
• Keywords:
  Blood-feeding Mosquitoes Salivary Glands Transcriptome
• Source:
  Insect Biochem Mol Biol. 95:1-9
• Pubmed ID:
  29526772
• Pubmed Central ID:
  PMC5927831
• Document Type:
  Journal Article
• Funding:
  R01 AI099231/AI/NIAID NIH HHSUnited States/ ; R01 CI000232/CI/NCPDCID CDC HHSUnited States/ ; T32 AI007414/AI/NIAID NIH HHSUnited States/ ; ZIA AI000810-19/Intramural NIH HHSUnited States/
• Volume:
  95
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:07be5a3ee823d76fd67d2d10896bcb50a7d389517aae74d06881b0001e57c796
• Download URL:
  https://stacks.cdc.gov/view/cdc/53692/cdc_53692_DS1.pdf
• File Type:
  [PDF - 402.74 KB ]