Detection of Spliced mRNA from Human Bocavirus 1 in Clinical Samples from Children with Respiratory Tract Infections

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Christensen, Andreas ; Døllner, Henrik ; Skanke, Lars Høsøien ; Krokstad, Sidsel ; Moe, Nina ; Nordbø, Svein Arne
• Description:
  Human bocavirus 1 (HBoV1) is a parvovirus associated with respiratory tract infections (RTIs) in children, but a causal relation has not yet been confirmed. To develop a qualitative reverse transcription PCR to detect spliced mRNA from HBoV1 and to determine whether HBoV1 mRNA correlated better with RTIs than did HBoV1 DNA, we used samples from HBoV1 DNA-positive children, with and without RTIs, to evaluate the test. A real-time reverse transcription PCR, targeting 2 alternatively spliced mRNAs, was developed. HBoV1 mRNA was detected in nasopharyngeal aspirates from 33 (25%) of 133 children with RTIs but in none of 28 controls (p<0.001). The analytical sensitivity and specificity of the test were good. Our data support the hypothesis that HBoV1 may cause RTIs, and we propose that HBoV1 mRNA could be used with benefit, instead of HBoV1 DNA, as a diagnostic target.
• Subjects:
  Child Child, Preschool Children DNA, Viral Female Human Bocavirus Humans Infant Male MRNA Nasopharynx Parvoviridae Infections Parvovirus Polymerase Chain Reaction Real-Time Polymerase Chain Reaction Research Respiratory Tract Infections Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger RNA, Viral RNA Splicing Sensitivity And Specificity Splicing Viruses

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• Source:
  Emerg Infect Dis. 19(4):574-580.
• Document Type:
  Journal Article
• Volume:
  19
• Issue:
  4
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:ee00b4ea90e4a298c1901bedb74601dba86a0728d9a3928add73dae7f94a3d45
• Download URL:
  https://stacks.cdc.gov/view/cdc/19038/cdc_19038_DS1.pdf
• File Type:
  [PDF - 405.18 KB ]