Detection of Infectious Poxvirus Particles

Nitsche, Andreas; Stern, Daniel; Ellerbrok, Heinz; Pauli, Georg

i

Detection of Infectious Poxvirus Particles

Supporting Files Public Domain

Jul 2006
By Nitsche, Andreas ; Stern, Daniel ; Ellerbrok, Heinz ; ...

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Nitsche, Andreas ; Stern, Daniel ; Ellerbrok, Heinz ; Pauli, Georg
Description:

To enable rapid and reliable detection of poxviruses in clinical and environmental specimens, a diagnostic approach was developed to detect <3 PFU of infectious poxvirus particles in <5 hours. This approach involved virus culture combined with real-time reverse transcription-polymerase chain reaction detection of 2 viral genes expressed immediately after infection.
Subjects:

Cell Culture Cell Line, Tumor Diagnostics Dispatch DNA, Viral Gene Expression Regulation, Viral Humans Orthopoxvirus Polymerase Chain Reaction Real-time PCR RNA, Messenger RNA, Viral Sensitivity And Specificity Time Factors Vaccinia Virus Virus Replication
Source:

Emerg Infect Dis. 12(7):1139-1141.
Document Type:

Journal Article
Volume:

12
Issue:

7
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:1a7cc1a6d3e437a436d09c71a7b27c038ff4e8dcc3ac0f9ecf975d9244182ad5
Download URL:

https://stacks.cdc.gov/view/cdc/16220/cdc_16220_DS1.pdf
File Type:

[PDF - 347.65 KB ]

ON THIS PAGE

Details Supporting Files

CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.

As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.