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Detection of Infectious Poxvirus Particles
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Jul 2006
Source: Emerg Infect Dis. 12(7):1139-1141.
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Alternative Title:Emerg Infect Dis
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Description:To enable rapid and reliable detection of poxviruses in clinical and environmental specimens, a diagnostic approach was developed to detect <3 PFU of infectious poxvirus particles in <5 hours. This approach involved virus culture combined with real-time reverse transcription-polymerase chain reaction detection of 2 viral genes expressed immediately after infection.
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