Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Nitsche, Andreas ; Stern, Daniel ; Ellerbrok, Heinz ; Pauli, Georg ;
Nitsche, Andreas ; Stern, Daniel ; Ellerbrok, Heinz ; Pauli, Georg ; Less -
Description:
To enable rapid and reliable detection of poxviruses in clinical and environmental specimens, a diagnostic approach was developed to detect <3 PFU of infectious poxvirus particles in <5 hours. This approach involved virus culture combined with real-time reverse transcription-polymerase chain reaction detection of 2 viral genes expressed immediately after infection.
Subject:
[+]
Cell Culture
Cell Line, Tumor
Diagnostics
Dispatch
DNA, Viral
Gene Expression Regulation, Viral
Humans
Orthopoxvirus
Polymerase Chain Reaction
Real-time PCR
RNA, Messenger
RNA, Viral
Sensitivity And Specificity
Time Factors
Vaccinia Virus
Virus Replication
Virus Replication

Source:
Emerg Infect Dis. 12(7):1139-1141. ;
Document Type:
Journal Article ;
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
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urn:sha256:1a7cc1a6d3e437a436d09c71a7b27c038ff4e8dcc3ac0f9ecf975d9244182ad5
File Type:

Supporting Files

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  	06-0093-F2.jpg	jpeg
  	06-0093.nxml	txt
  	license.txt	txt

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