Rapid detection and typing of pathogenic nonpneumophila Legionella spp. isolates using a multiplex real-time PCR assay

Benitez, Alvaro J.; Winchell, Jonas M.

doi:10.1016/j.diagmicrobio.2016.01.007

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CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners. As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.

i

Rapid detection and typing of pathogenic nonpneumophila Legionella spp. isolates using a multiplex real-time PCR assay

4 2016
By Benitez, Alvaro J. ; Winchell, Jonas M.
http://dx.doi.org/10.1016/j.diagmicrobio.2016.01.007
Source: Diagn Microbiol Infect Dis. 84(4):298-303

[PDF-733.36 KB]

English

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Details:

Alternative Title:

Diagn Microbiol Infect Dis
Personal Author:

Benitez, Alvaro J. ; Winchell, Jonas M.

Benitez, Alvaro J. ; Winchell, Jonas M. Less -
Description:

We developed a single tube multiplex real-time PCR assay that allows for the rapid detection and typing of 9 nonpneumophila Legionella spp. isolates that are clinically relevant. The multiplex assay is capable of simultaneously detecting and discriminating L. micdadei, L. bozemanii, L. dumoffii, L. longbeachae, L. feeleii, L. anisa, L. parisiensis, L. tucsonensis serogroup (sg) 1 and 3, and L. sainthelensis sg 1 and 2 isolates. Evaluation of the assay with nucleic acid from each of these species derived from both clinical and environmental isolates and typing strains demonstrated 100% sensitivity and 100% specificity when tested against 43 other Legionella spp. Typing of L. anisa, L. parisiensis, and L. tucsonensis sg 1 and 3 isolates was accomplished by developing a real-time PCR assay followed by high-resolution melt (HRM) analysis targeting the ssrA gene. Further typing of L. bozemanii, L. longbeachae, and L. feeleii isolates to the serogroup level was accomplished by developing a real-time PCR assay followed by HRM analysis targeting the mip gene. When used in conjunction with other currently available diagnostic tests, these assays may aid in rapidly identifying specific etiologies associated with Legionella outbreaks, clusters, sporadic cases, and potential environmental sources.
Subjects:

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Humans Legionella Legionellosis Multiplex Polymerase Chain Reaction Real-Time Polymerase Chain Reaction Sensitivity And Specificity Time Factors
Keywords:

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Detection HRM Analysis Legionella Spp. Real-time PCR Typing
Source:

Diagn Microbiol Infect Dis. 84(4):298-303
Pubmed ID:

26867966
Pubmed Central ID:

PMC8972187
Document Type:

Journal Article
Funding:

CC999999/ImCDC/Intramural CDC HHSUnited States/
Volume:

84
Issue:

4
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha256:8c6693c2e3fbaa611687427b10a7b54534d2ffa7997176e12b6ebe664f032e04
Download URL:

https://stacks.cdc.gov/view/cdc/115875/cdc_115875_DS1.pdf
File Type:

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