Details:

Alternative Title:
BMC Genomics
Personal Author:
Go, Jennifer T ; Belisle, Sarah E ; Tchitchek, Nicolas ; Tumpey, Terrence M ; Ma, Wenjun ; ... More +
Go, Jennifer T ; Belisle, Sarah E ; Tchitchek, Nicolas ; Tumpey, Terrence M ; Ma, Wenjun ; Richt, Juergen A ; Safronetz, David ; Feldmann, Heinz ; Katze, Michael G Less -
Description:
Background

The 2009 pandemic H1N1 influenza virus emerged in swine and quickly became a major global health threat. In mouse, non human primate, and swine infection models, the pH1N1 virus efficiently replicates in the lung and induces pro-inflammatory host responses; however, whether similar or different cellular pathways were impacted by pH1N1 virus across independent infection models remains to be further defined. To address this we have performed a comparative transcriptomic analysis of acute phase responses to a single pH1N1 influenza virus, A/California/04/2009 (CA04), in the lung of mice, macaques and swine.

Results

Despite similarities in the clinical course, we observed differences in inflammatory molecules elicited, and the kinetics of their gene expression changes across all three species. We found genes associated with the retinoid X receptor (RXR) signaling pathway known to control pro-inflammatory and metabolic processes that were differentially regulated during infection in each species, though the heterodimeric RXR partner, pathway associated signaling molecules, and gene expression patterns varied among the three species.

Conclusions

By comparing transcriptional changes in the context of clinical and virological measures, we identified differences in the host transcriptional response to pH1N1 virus across independent models of acute infection. Antiviral resistance and the emergence of new influenza viruses have placed more focus on developing drugs that target the immune system. Underlying overt clinical disease are molecular events that suggest therapeutic targets identified in one host may not be appropriate in another.


Subjects:
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Animals Female Gene Expression Profiling Host-Pathogen Interactions Host Specificity Influenza A Virus, H1N1 Subtype Lung Macaca Mice Mice, Inbred BALB C Orthomyxoviridae Infections Pandemics Protein Multimerization Retinoid X Receptors Signal Transduction Swine Transcriptome
Source:
BMC Genomics. 2012; 13:627.
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:cf36f7a26180e8daca0b64a12ef7b7c9d1c3d82a786f99e1ce55eae2d8a497d4
Download URL:
https://stacks.cdc.gov/view/cdc/12143/cdc_12143_DS1.pdf
File Type:

Supporting Files

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