Quantification of DEET and neonicotinoid pesticide biomarkers in human urine by online solid-phase extraction high-performance liquid chromatography-tandem mass spectrometry

Details

• Alternative Title:
  Anal Bioanal Chem
• Personal Author:
  Baker, Samuel E. ; Serafim, Amanda Bishop ; Morales-Agudelo, Pilar ; Vidal, Meghan ; Calafat, Antonia M. ; Ospina, Maria
• Description:
  Neonicotinoid insecticides are widely used replacements for organophosphate and carbamate insecticides, but the extent of human exposure is largely unknown. On the other hand, based on urinary concentrations of DEET metabolites, human exposure to N,N-diethyl-m-toluamide (DEET) appears to be widespread. We developed a fast online solid-phase extraction high-performance liquid chromatography-isotope dilution tandem mass spectrometry (HPLC-MS/MS) method to measure in 200 μL of human urine the concentrations of six neonicotinoid biomarkers (acetamiprid, N-desmethyl-acetamiprid, clothianidin, imidacloprid, 5-hydroxy-imidacloprid, thiacloprid), and two DEET biomarkers (3-diethyl-carbamoyl benzoic acid, 3-ethyl-carbamoyl benzoic acid). Limits of detection ranged from 0.01 to 0.1 μg/L, depending on the biomarker. Accuracy ranged from 91 to 116% and precision ranged from 3.7 to 10 %RSD. The presented method can be used to increase our understanding of exposure to neonicotinoid insecticides and DEET, and to evaluate the potential health effects from such exposures.
• Subjects:
  Article Biomarkers Chromatography, High Pressure Liquid DEET Female HPLC-MS/MS Humans Insecticides Insect Repellents Limit Of Detection Male N,N-Diethyl-m-toluamide Neonicotinoids Online SPE Quality Control Reference Standards Reproducibility Of Results Solid Phase Extraction Tandem Mass Spectrometry

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• Source:
  Anal Bioanal Chem. 411(3):669-678
• Pubmed ID:
  30483854
• Pubmed Central ID:
  PMC6691966
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHS/United States
• Volume:
  411
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:b16bd7111946bf8694b4e1c3293c87782ad8ee29d119083002976013520d004a
• Download URL:
  https://stacks.cdc.gov/view/cdc/80673/cdc_80673_DS1.pdf
• File Type:
  [PDF - 739.18 KB ]