Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Daum, Luke T. ; Shaw, Michael W. ; Klimov, Alexander I. ; Canas, Linda C. ; Macias, Elizabeth A. ; ... More +
Daum, Luke T. ; Shaw, Michael W. ; Klimov, Alexander I. ; Canas, Linda C. ; Macias, Elizabeth A. ; Niemeyer, Debra ; Chambers, James P. ; Renthal, Robert ; Shrestha, Sanjaya K. ; Acharya, Ramesh P. ; Huzdar, Shankar P. ; Rimal, Nirmal ; Myint, Khin S. ; Gould, Philip ; Less -
Description:
In July 2004, an outbreak of influenza A (H3N2) was detected at 3 Bhutanese refugee camps in southeastern Nepal. Hemagglutination inhibition showed that approximately 40% of the viruses from this outbreak were antigenically distinct from the A/Wyoming/3/03 vaccine strain. Four amino acid differences were observed in most of the 26 isolates compared with the A/Wyoming/3/2003 vaccine strain. All 4 substitutions are located within or adjacent to known antibody-binding sites. Several isolates showed a lysine-to-asparagine substitution at position 145 (K145N) in the hemagglutinin molecule, which may be noteworthy since position 145 is located within a glycosylation site and adjacent to an antibody-binding site. H3N2 viruses continue to drift from the vaccine strain and may remain as the dominant strains during the 2005-2006 influenza season. Thus, the 2005-2006 Northern Hemisphere vaccine strain was changed to A/California/7/2004, a virus with all 4 amino acid substitutions observed in these Nepalese isolates.
Subject:
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Amino Acid Sequence
Antigenic Variation
Child
Disease Outbreaks
Female
Genetic Drift
H3N2
Hemagglutination Inhibition Tests
Hemagglutinin
Hemagglutinins
Humans
Influenza, Human
Influenza A Virus
Influenza A Virus, H3N2 Subtype
Keywords: Influena A
Male
Models, Molecular
Molecular Sequence Data
Nepal
Phylogeny
Research
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral
Sequence Alignment
Sequence Analysis, DNA
Variant

Source:
Emerg Infect Dis. 11(8):1186-1191.
Document Type:
Journal Article ;
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:8ba4e69272d50144251e91c85d3b4859ae23da40da29d8aa47ef878e0151cc73
File Type:

Supporting Files

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