i
Structural and Functional Studies of Membrane Remodeling Machines
-
Apr 08 2015
-
Source: Methods Cell Biol. 128:165-200.
[PDF-1.73 MB]
Details:
-
Alternative Title:Methods Cell Biol
-
Personal Author:
-
Description:Building cells from their component parts will hinge upon our ability to reconstitute biochemical compartmentalization and exchange between membrane-delimited organelles. By contrast with our understanding of other cellular events, the mechanisms that govern membrane trafficking has lagged because the presence of phospholipid bilayers complicates the use of standard methods. This chapter describes in vitro methods for purifying, reconstituting, and visualizing membrane remodeling activities directly by electron cryomicroscopy.
-
Subjects:
-
Source:
-
Pubmed ID:25997348
-
Pubmed Central ID:PMC6089609
-
Document Type:
-
Funding:
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
