Details:

Alternative Title:
Science
Publisher's site:
http://dx.doi.org/10.1126/science.aad8305
Personal Author:
McCullough, John ; Clippinger, Amy K. ; Talledge, Nathaniel ; Skowyra, Michael L. ; Saunders, Marissa G. ; ... More +
McCullough, John ; Clippinger, Amy K. ; Talledge, Nathaniel ; Skowyra, Michael L. ; Saunders, Marissa G. ; Naismith, Teresa V. ; Colf, Leremy A. ; Afonine, Pavel ; Arthur, Christopher ; Sundquist, Wesley I. ; Hanson, Phyllis I. ; Frost, Adam ; Less -
Description:
The endosomal sorting complexes required for transport (ESCRT) proteins mediate fundamental membrane remodeling events that require stabilizing negative membrane curvature. These include endosomal intralumenal vesicle formation, HIV budding, nuclear envelope closure, and cytokinetic abscission. ESCRT-III subunits perform key roles in these processes by changing conformation and polymerizing into membrane-remodeling filaments. Here, we report the 4 angstrom resolution cryogenic electron microscopy reconstruction of a one-start, double-stranded helical copolymer composed of two different human ESCRT-III subunits, charged multivesicular body protein 1B (CHMP1B) and increased sodium tolerance 1 (IST1). The inner strand comprises "open" CHMP1B subunits that interlock in an elaborate domain-swapped architecture and is encircled by an outer strand of "closed" IST1 subunits. Unlike other ESCRT-III proteins, CHMP1B and IST1 polymers form external coats on positively curved membranes in vitro and in vivo. Our analysis suggests how common ESCRT-III filament architectures could stabilize different degrees and directions of membrane curvature.
Subject:
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Article
Biopolymers
Cell Membrane
Cryoelectron Microscopy
Endosomal Sorting Complexes Required For Transport
Humans
Oncogene Proteins
Protein Structure, Secondary
Protein Structure, Tertiary

Source:
Science. 350(6267):1548-1551.
Pubmed ID:
26634441
Pubmed Central ID:
PMC4684769
Document Type:
Journal Article ;
Funding:
1DP2GM110772-01/DP/NCCDPHP CDC HHS/United States ; 1P01 GM063210/GM/NIGMS NIH HHS/United States ; 2P50GM082545-06/GM/NIGMS NIH HHS/United States ; DP2 GM110772/GM/NIGMS NIH HHS/United States ; P01 GM063210/GM/NIGMS NIH HHS/United States ; ... More +
1DP2GM110772-01/DP/NCCDPHP CDC HHS/United States ; 1P01 GM063210/GM/NIGMS NIH HHS/United States ; 2P50GM082545-06/GM/NIGMS NIH HHS/United States ; DP2 GM110772/GM/NIGMS NIH HHS/United States ; P01 GM063210/GM/NIGMS NIH HHS/United States ; P41 RR17573/RR/NCRR NIH HHS/United States ; P50 GM082545/GM/NIGMS NIH HHS/United States ; R01 AI051174/AI/NIAID NIH HHS/United States ; R01AI051174/AI/NIAID NIH HHS/United States ; R01GM076686/GM/NIGMS NIH HHS/United States ; R01GM112080/GM/NIGMS NIH HHS/United States ; R01NS050717/NS/NINDS NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:f4cadab81953a3f0ce8e739868c5f4aa28ad5b182546614b1251a8afffca70e4
File Type:

Supporting Files

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