Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay

Burkhalter, Kristen L.; Savage, Harry M.

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Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay

Supporting Files Public Domain

Apr 15 2017
By Burkhalter, Kristen L. ; Savage, Harry M.

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Burkhalter, Kristen L. ; Savage, Harry M.
Description:

We assayed Zika virus-infected mosquitoes stored at room temperature for <30 days for live virus by using plaque assay and virus RNA by using real-time reverse transcription PCR. Viable virus was detected in samples stored <10 days, and virus RNA was detected in samples held for 30 days.
Subjects:

Aedes Animals Chlorocebus Aethiops Desiccation Dispatch Female Humans Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Rna, Viral Vero Cells Viral Plaque Assay Zika Virus
Source:

Emerg Infect Dis. 23(4):680-681.
Pubmed ID:

28075325
Pubmed Central ID:

PMC5367413
Document Type:

Journal Article
Volume:

23
Issue:

4
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:fcb57ecc832a88db88a93c93eb1705bc10ac7b314d0e6d891319777c3d580c56
Download URL:

https://stacks.cdc.gov/view/cdc/45040/cdc_45040_DS1.pdf
File Type:

[PDF - 349.47 KB ]

File Language:

English

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