Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil

Details

• Alternative Title:
  Arch Virol
• Personal Author:
  Pauvolid-Corrêa, Alex ; Solberg, Owen ; Couto-Lima, Dinair ; Kenney, Joan ; Serra-Freire, Nicolau ; Brault, Aaron ; Nogueira, Rita ; Langevin, Stanley ; Komar, Nicholas
• Description:
  We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex-borne flaviviruses amplified by birds and the insect-only flaviviruses.
• Subjects:
  Animals Article Brazil Cercopithecus Aethiops Culex Female Flavivirus Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Ticks Vero Cells

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• Source:
  Arch Virol. 160(1):21-27.
• Pubmed ID:
  25252815
• Pubmed Central ID:
  PMC4785999
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  160
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:56c486f0d9c6a3b055dc4c6de790187dd5f10eb04264b4dc2a853c7793159efb
• Download URL:
  https://stacks.cdc.gov/view/cdc/38409/cdc_38409_DS1.pdf
• File Type:
  [PDF - 853.83 KB ]