Details:

Alternative Title:
Arch Virol
Publisher's site:
http://dx.doi.org/10.1007/s00705-014-2219-8
Personal Author:
Pauvolid-Corrêa, Alex ; Solberg, Owen ; Couto-Lima, Dinair ; Kenney, Joan ; Serra-Freire, Nicolau ; ... More +
Pauvolid-Corrêa, Alex ; Solberg, Owen ; Couto-Lima, Dinair ; Kenney, Joan ; Serra-Freire, Nicolau ; Brault, Aaron ; Nogueira, Rita ; Langevin, Stanley ; Komar, Nicholas ; Less -
Description:
We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex-borne flaviviruses amplified by birds and the insect-only flaviviruses.
Subject:
[+]
Animals
Article
Cercopithecus Aethiops
Culex
Female
Flavivirus
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral
Ticks
Vero Cells

Source:
Arch Virol. 160(1):21-27. ;
Pubmed ID:
25252815
Pubmed Central ID:
PMC4785999
Document Type:
Journal Article ;
Funding:
CC999999/Intramural CDC HHS/United States ;
Place as Subject:
Brazil ;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:56c486f0d9c6a3b055dc4c6de790187dd5f10eb04264b4dc2a853c7793159efb
File Type:

Supporting Files

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