Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes

Caschera, Filippo; Lee, Jin Woo; Ho, Kenneth K. Y.; Liu, Allen P.; Jewett, Michael C.

doi:10.1039/c6cc00223d

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Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes

Published Date:

Apr 07 2016

Publisher's site:

http://dx.doi.org/10.1039/c6cc00223d

Source:

Chem Commun (Camb). 52(31):5467-5469.

[PDF-413.59 KB]

Details:

Alternative Title:

Chem Commun (Camb)

Personal Author:

Caschera, Filippo ; Lee, Jin Woo ; Ho, Kenneth K. Y. ; Liu, Allen P. ; Jewett, Michael C. ;

Description:

A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was analysed using spinning disk confocal microscopy. Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells.

Subject:

[+]

Pubmed ID:

27019994

Pubmed Central ID:

PMC4829377

Document Type:

Journal Article

Funding:

DP2 HL117748/HL/NHLBI NIH HHS/United States ; T32 EB005582/EB/NIBIB NIH HHS/United States ; DP2 HL117748-01/DP/NCCDPHP CDC HHS/United States ; T32EB005582/EB/NIBIB NIH HHS/United States ;

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

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Supporting Files:

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	nihms773485f1.jpg	image/jpeg
	nihms773485f2.gif	image/gif
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Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes

Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes

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