Details:

Alternative Title:
Chem Commun (Camb)
Publisher's site:
http://dx.doi.org/10.1039/c6cc00223d
Personal Author:
Caschera, Filippo ; Lee, Jin Woo ; Ho, Kenneth K. Y. ; Liu, Allen P. ; Jewett, Michael C.
Caschera, Filippo ; Lee, Jin Woo ; Ho, Kenneth K. Y. ; Liu, Allen P. ; Jewett, Michael C. Less -
Description:
A cell-free expression platform for making bacterial ribosomes encapsulated within giant liposomes was capable of synthesizing sfGFP. The liposomes were prepared using a double emulsion template, and compartmentalized in vitro protein synthesis was analysed using spinning disk confocal microscopy. Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells.
Subject:
[+]
Article
Cell-Free System
Emulsions
Escherichia Coli
Green Fluorescent Proteins
Liposomes
Phospholipids
Protein Biosynthesis
Ribosomes

Source:
Chem Commun (Camb). 52(31):5467-5469.
Pubmed ID:
27019994
Pubmed Central ID:
PMC4829377
Document Type:
Journal Article
Funding:
DP2 HL117748/HL/NHLBI NIH HHS/United States ; T32 EB005582/EB/NIBIB NIH HHS/United States ; DP2 HL117748-01/DP/NCCDPHP CDC HHS/United States ; T32EB005582/EB/NIBIB NIH HHS/United States
DP2 HL117748/HL/NHLBI NIH HHS/United States ; T32 EB005582/EB/NIBIB NIH HHS/United States ; DP2 HL117748-01/DP/NCCDPHP CDC HHS/United States ; T32EB005582/EB/NIBIB NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:94f95916cf2e97c2ff78f4d03d3498edcf0005cf012f56ca971d88d546a4a9ce
File Type:

Supporting Files

• 	NIHMS773485-supplement-ESI.docx	docx
  	nihms773485.nxml	bin
  	nihms773485f1.gif	gif
  	nihms773485f1.jpg	jpeg
  	nihms773485f2.gif	gif
  	nihms773485f2.jpg	jpeg

More +