An amacrine cell circuit for signaling steady illumination in the retina

Details

• Alternative Title:
  Cell Rep
• Personal Author:
  Jacoby, Jason ; Zhu, Yongling ; DeVries, Steven H. ; Schwartz, Gregory
• Description:
  Decades of research have focused on the circuit connectivity between retinal neurons, but only a handful of amacrine cells have been described functionally and placed in the context of a specific retinal circuit. Here, we identify a circuit where inhibition from a specific amacrine cell plays a vital role in shaping the feature selectivity of a postsynaptic ganglion cell. We record from transgenically labeled CRH-1 amacrine cells and identify a postsynaptic target for CRH-1 amacrine cell inhibition in an atypical retinal ganglion cell (RGC) in mouse retina, the Suppressed-by-Contrast (SbC) RGC. Unlike other RGC types, SbC RGCs spike tonically in steady illumination and are suppressed by both increases and decreases in illumination. Inhibition from GABAergic CRH-1 amacrine cells shapes this unique contrast response profile to positive contrast. We show the existence and impact of this circuit, with both paired recordings and cell-type-specific ablation.
• Subjects:
  Amacrine Cells Animals Mice Mice, Transgenic Photic Stimulation Retina Retinal Ganglion Cells Signal Transduction Synaptic Transmission
• Source:
  Cell Rep. 13(12):2663-2670.
• Pubmed ID:
  26711334
• Pubmed Central ID:
  PMC4698005
• Document Type:
  Journal Article
• Funding:
  1F32EY025930-01/EY/NEI NIH HHS/United States ; DP2 EY026770/EY/NEI NIH HHS/United States ; F32 EY025930/EY/NEI NIH HHS/United States ; NIH DP2-DEY026770A/DP/NCCDPHP CDC HHS/United States ; R01 EY018204/EY/NEI NIH HHS/United States
• Volume:
  13
• Issue:
  12
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:f4c2553e6839f05aea75c8eec4a170ce2b8126451890448b6574a5f5feff5136
• Download URL:
  https://stacks.cdc.gov/view/cdc/39042/cdc_39042_DS1.pdf
• File Type:
  [PDF - 903.75 KB ]