αβ T Cells and a Mixed Th1/Th17 Response are Important in Organic Dust-Induced Airway Disease

Poole, Jill A.; Gleason, Angela M.; Bauer, Christopher; West, William W.; Alexis, Neil; Reynolds, Stephen J.; Romberger, Debra J.; Kielian, Tammy

doi:10.1016/j.anai.2012.06.015

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

αβ T Cells and a Mixed Th1/Th17 Response are Important in Organic Dust-Induced Airway Disease

Published Date:

Jul 24 2012

Publisher's site:

http://dx.doi.org/10.1016/j.anai.2012.06.015 New Site Icon

Source:

Ann Allergy Asthma Immunol. 109(4):266-273.e2.

[PDF-933.22 KB]

Details:

Personal Authors:

Poole, Jill A. ; Gleason, Angela M. ; Bauer, Christopher ; West, William W. ; Alexis, Neil ; ... More ▼

Keywords:

[+]

Pubmed ID:

23010233

Pubmed Central ID:

PMC4019222

Description:

Organic dust exposure in agricultural environments induces an inflammatory response that attenuates over time, yet repetitive dust exposures result in chronic lung diseases. Animal models resembling this chronic lung inflammatory response have been developed, yet the underlying cellular mechanisms are not well defined.|Because mice repetitively exposed to organic dust extracts (DE) display increased CD3+ T cell lung infiltrates, we sought to determine the phenotype and importance of these cells.|Mice received swine confinement DE repetitively for 3 weeks by established intranasal inhalation protocol. Studies were conducted with peptidoglycan (PGN) because it is a major DE component in large animal farming environments and has shared similar biologic effects with DE. Enumeration of T cells and intracellular cytokine profiles were conducted by flow cytometry techniques. Whole lung homogenate cytokines were analyzed by multiplex immunoassay. T cell receptor (TCR) αβ knockouts were used to determine the functional importance of αβ-expressing T cells.|DE increased lung-associated CD3+CD4+ T cells and interleukin (IL)-17 (but not IL-4, interferon [IFN]-γ, IL-10) producing CD4+ T cells. PGN treatment resulted in increased IL-17 and IFN-γ producing CD4+ T cells and IFN-γ producing CD8+ T cells. Both DE and PGN augmented expression of cytokines associated with Th1 and Th17 polarization in lung homogenates. DE-induced lung mononuclear aggregates and bronchiolar compartment inflammation were significantly reduced in TCR knockout animals; however, neutrophil influx and alveolar compartment inflammation were not affected.|Studies demonstrated that DE and PGN exposure promote a Th1/Th17 lung microenvironment and that αβ-expressing T cells are important in mediating DE-induced lung pathologic conditions.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

1R01OH008539-01/OH/NIOSH CDC HHS/United States
3R01NS040730/NS/NINDS NIH HHS/United States
5U50 OH008085/OH/NIOSH CDC HHS/United States
ES015522-03S1/ES/NIEHS NIH HHS/United States
K08 ES015522/ES/NIEHS NIH HHS/United States
K08 ES015522-01/ES/NIEHS NIH HHS/United States
R01 ES019325/ES/NIEHS NIH HHS/United States
R01 ES019325/ES/NIEHS NIH HHS/United States
R01 NS040730/NS/NINDS NIH HHS/United States

Main Document Checksum:

[+]

Supporting Files:

	nihms491739f4.gif	image/gif
	nihms491739f4.jpg	image/jpeg
	nihms491739f5.gif	image/gif
	nihms491739f5.jpg	image/jpeg
	NIHMS491739-supplement-supplement_1.pdf	application/pdf
	nihms491739.nxml	application/octet-stream
	nihms491739f1.gif	image/gif
	nihms491739f1.jpg	image/jpeg
	nihms491739f2.gif	image/gif
	nihms491739f2.jpg	image/jpeg
	nihms491739f3.gif	image/gif
	nihms491739f3.jpg	image/jpeg

No Related Documents.

You May Also Like:

Pattern Recognition Scavenger Receptor A/CD204 Regulates Airway Inflammatory Homeostasis Following Organic Dust Extract Exposures

Personal Author:

Poole, Jill A.; Anderson, Leigh; Gleason, Angela M.; West, William W.; Romberger, Debra J.; Wyatt, Todd A.;

Published:

2015 Jan-Mar

Source:

J Immunotoxicol. 12(1):64-73.

Description:

Exposure to agriculture organic dusts, comprised of a diversity of pathogen-associated molecular patterns, results in chronic airway diseases. The multi-functional class A macrophage scavenger receptor (SRA)/CD204 has emerged as an important class of...

File Type:

[PDF - 874.93 KB]
Influence of farming exposure on the development of asthma and asthma-like symptoms

Personal Author:

Wells, Adam D.; Poole, Jill A.; Romberger, Debra J.;

Published:

Jul 31 2014

Source:

Int Immunopharmacol. 2014; 23(1):356-363.

Description:

Based upon age and type of farming exposures, a wide range of studies demonstrate either protective or deleterious effects of the farming environment on asthma. In this review, we highlight key studies supporting the concept that farming exposure pro...

File Type:

[PDF - 868.43 KB]
Respiratory Health Effects of Large Animal Farming Environments

Personal Author:

May, Sara; Romberger, Debra J.; Poole, Jill A.;

Published:

2012

Source:

J Toxicol Environ Health B Crit Rev. 15(8):524-541.

Description:

With increases in large animal-feeding operations to meet consumer demand, adverse upper and lower respiratory health effects in exposed agriculture workers are a concern. The aim of this study was to review large animal confinement feeding operation...

File Type:

[PDF - 322.76 KB]
Post-injury and resolution response to repetitive inhalation exposure to agricultural organic dust in mice

Personal Author:

Warren, Kristi J.; Wyatt, Todd A.; Romberger, Debra J.; Ailts, Isaak; West, William W.; Nelson, Amy J.; Nordgren, Tara M.; Staab, Elizabeth; Heires, Art J.; Poole, Jill A.;

Published:

Feb 21 2017

Source:

Safety (Basel). 3(1).

Description:

Inhalation of organic dusts in agricultural environments causes airway inflammatory diseases. Despite advances in understanding the airway response to dust-induced inflammation, less is known about the transition from lung injury to repair and recove...

File Type:

[PDF - 791.51 KB]
The Effect of Inhalant Organic Dust on Bone Health

Personal Author:

Carrington, Joseph M; Poole, Jill A;

Published:

Feb 22 2018

Source:

Curr Allergy Asthma Rep. 18(3):16

Description:

Purpose of Review: : Agriculture remains a major economic sector globally, and workers experience high rates of chronic inflammatory lung and musculoskeletal diseases. Whereas obstructive pulmonary diseases are known risk factors for bone loss, the u...

File Type:

[PDF - 710.02 KB]