Pattern Recognition Scavenger Receptor A/CD204 Regulates Airway Inflammatory Homeostasis Following Organic Dust Extract Exposures

Details

• Alternative Title:
  J Immunotoxicol
• Personal Author:
  Poole, Jill A. ; Anderson, Leigh ; Gleason, Angela M. ; West, William W. ; Romberger, Debra J. ; Wyatt, Todd A.
• Description:
  Exposure to agriculture organic dusts, comprised of a diversity of pathogen-associated molecular patterns, results in chronic airway diseases. The multi-functional class A macrophage scavenger receptor (SRA)/CD204 has emerged as an important class of pattern recognition receptors with broad ligand binding ability. The objective was to determine the role of SRA in mediating repetitive and post-inflammatory organic dust extract (ODE)-induced airway inflammation. Wild-type (WT) and SRA knockout (KO) mice were intra-nasally treated with ODE or saline daily for 3 weeks and immediately euthanized or allowed to recover for 1 week. Results show that lung histopathologic changes were increased in SRA KO mice as compared to WT following repetitive ODE exposures marked predominately by increased size and distribution of lymphoid aggregates. After a 1-week recovery from daily ODE treatments, there was significant resolution of lung injury in WT mice, but not SRA KO animals. The increased lung histopathology induced by ODE treatment was associated with decreased accumulation of neutrophils, but greater accumulation of CD4(+) T-cells. The lung cytokine milieu induced by ODE was consistent with a TH1/TH17 polarization in both WT and SRA KO mice. Overall, the data demonstrate that SRA/CD204 plays an important role in the normative inflammatory lung response to ODE, as evidenced by the enhanced dust-mediated injury viewed in the absence of this receptor.
• Subjects:
  Agricultural Workers' Diseases Animals Antigens, Bacterial Antigens, Fungal Cytokines Dust Homeostasis Humans Mice Mice, Inbred C57BL Mice, Knockout Occupational Exposure Pneumonia Receptors, Pattern Recognition Respiratory System Scavenger Receptors, Class A Th1 Cells Th17 Cells

  More +
• Keywords:
  Farm Inflammation Lung Lymphocyte TH17 Toll-like Receptor
• Source:
  J Immunotoxicol. 12(1):64-73
• Pubmed ID:
  24491035
• Pubmed Central ID:
  PMC4119855
• Document Type:
  Journal Article
• Funding:
  R01ES019325/ES/NIEHS NIH HHSUnited States/ ; R01 AA017993/AA/NIAAA NIH HHSUnited States/ ; R01 ES019325/ES/NIEHS NIH HHSUnited States/ ; U54OH010162/OH/NIOSH CDC HHSUnited States/ ; U54 OH010162/OH/NIOSH CDC HHSUnited States/ ; R01AA017993/AA/NIAAA NIH HHSUnited States/ ; R01OH008539/OH/NIOSH CDC HHSUnited States/ ; R01 OH008539/OH/NIOSH CDC HHSUnited States/
• Volume:
  12
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:188607e503240a0b675c09685fa0c0effba1a2d35c16d1f4c24b3a4da8bfa3d99115e93d85ccbaec8f95beb7231edb387739a1d4ba2f6b6007bba3a118da7bc8
• Download URL:
  https://stacks.cdc.gov/view/cdc/30061/cdc_30061_DS1.pdf
• File Type:
  [PDF - 874.93 KB ]