Details:

Alternative Title:
Virology
Personal Author:
Guo, Rong ; Meskauskas, Arturas ; Dinman, Jonathan D. ; Simon, Anne E.
Guo, Rong ; Meskauskas, Arturas ; Dinman, Jonathan D. ; Simon, Anne E. Less -
Description:
SatC is a noncoding subviral RNA associated with Turnip crinkle virus (TCV). A 100-nt stretch in the 3' UTR of TCV contains three hairpins and two pseudoknots that fold into a tRNA-shaped structure (TSS) that binds 80S ribosomes. The 3' half of satC is derived from TCV and contains 6-nt differences in the TSS-analogous region. SatC binds poorly to 80S ribosomes, and molecular modeling that predicted the 3D structure of the TSS did not predict a similar structure for satC. When the satC TSS region was step-wise converted to the original TCV TSS bases, ribosome binding increased to TCV TSS levels without significantly affecting satC replication. However, mutant satC was less fit when accumulating in plants and gave rise to numerous second site changes that weakened one of two satC conformations. These results suggest that minor changes from the original TCV sequence in satC reflect requirements other than elimination of ribosome binding.
Subjects:
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Article Carmovirus Evolution, Molecular Helper Viruses Models, Molecular Nucleic Acid Conformation Plants Ribosomes RNA, Satellite RNA, Untranslated RNA Conformational Shift RNA Evolution RNA Structure/function Satellite RNA
Source:
Virology. 2011; 419(1):10-16
Pubmed ID:
21862095
Pubmed Central ID:
PMC3176665
Document Type:
Journal Article
Funding:
T32 AI051967/AI/NIAID NIH HHS/United States ; 2T32AI051967-06A1/AI/NIAID NIH HHS/United States ; GM 061515-05A2/G120CD1/CD/ODCDC CDC HHS/United States ; GM R01058859/GM/NIGMS NIH HHS/United States ; R01 GM061515-08/GM/NIGMS NIH HHS/United States ; ... More +
T32 AI051967/AI/NIAID NIH HHS/United States ; 2T32AI051967-06A1/AI/NIAID NIH HHS/United States ; GM 061515-05A2/G120CD1/CD/ODCDC CDC HHS/United States ; GM R01058859/GM/NIGMS NIH HHS/United States ; R01 GM061515-08/GM/NIGMS NIH HHS/United States ; R01 GM061515/GM/NIGMS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:f6115d5c5c342f7b885f169b134b0665a1621ef9e7b7de2706116d9e047760d9
Download URL:
https://stacks.cdc.gov/view/cdc/33246/cdc_33246_DS1.pdf
File Type:

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