Details:

Alternative Title:
Nat Chem Biol
Personal Author:
Kubota, Miles ; Tran, Catherine ; Spitale, Robert C
Kubota, Miles ; Tran, Catherine ; Spitale, Robert C Less -
Description:
Proper gene expression is essential for the survival of every cell. Once thought to be a passive transporter of genetic information, RNA has recently emerged as a key player in nearly every pathway in the cell. A full description of its structure is critical to understanding RNA function. Decades of research have focused on utilizing chemical tools to interrogate the structures of RNAs, with recent focus shifting to performing experiments inside living cells. This Review will detail the design and utility of chemical reagents used in RNA structure probing. We also outline how these reagents have been used to gain a deeper understanding of RNA structure in vivo. We review the recent merger of chemical probing with deep sequencing. Finally, we outline some of the hurdles that remain in fully characterizing the structure of RNA inside living cells, and how chemical biology can uniquely tackle such challenges.
Subject:
[+]
Article
Humans
Models, Molecular
Molecular Probes
Nucleic Acid Conformation
RNA

Source:
Nat Chem Biol. 11(12):933-941.
Pubmed ID:
26575240
Pubmed Central ID:
PMC5068366
Document Type:
Journal Article
Funding:
DP2 GM119164/GM/NIGMS NIH HHS/United States ; 1DP2GM119164-01/DP/NCCDPHP CDC HHS/United States
DP2 GM119164/GM/NIGMS NIH HHS/United States ; 1DP2GM119164-01/DP/NCCDPHP CDC HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:3a7eb08ce9684e5d62adbd6de1994ec1b0856fd81d412829cc5bfc852c6f2d72
File Type:

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