Progress and challenges for chemical probing of RNA structure inside living cells

Details

• Alternative Title:
  Nat Chem Biol
• Personal Author:
  Kubota, Miles ; Tran, Catherine ; Spitale, Robert C
• Description:
  Proper gene expression is essential for the survival of every cell. Once thought to be a passive transporter of genetic information, RNA has recently emerged as a key player in nearly every pathway in the cell. A full description of its structure is critical to understanding RNA function. Decades of research have focused on utilizing chemical tools to interrogate the structures of RNAs, with recent focus shifting to performing experiments inside living cells. This Review will detail the design and utility of chemical reagents used in RNA structure probing. We also outline how these reagents have been used to gain a deeper understanding of RNA structure in vivo. We review the recent merger of chemical probing with deep sequencing. Finally, we outline some of the hurdles that remain in fully characterizing the structure of RNA inside living cells, and how chemical biology can uniquely tackle such challenges.
• Subjects:
  Humans Models, Molecular Molecular Probes Nucleic Acid Conformation RNA
• Source:
  Nat Chem Biol. 11(12):933-941
• Pubmed ID:
  26575240
• Pubmed Central ID:
  PMC5068366
• Document Type:
  Journal Article
• Funding:
  DP2 GM119164/GM/NIGMS NIH HHSUnited States/ ; 1DP2GM119164-01/DP/NCCDPHP CDC HHSUnited States/
• Volume:
  11
• Issue:
  12
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:3a7eb08ce9684e5d62adbd6de1994ec1b0856fd81d412829cc5bfc852c6f2d72
• Download URL:
  https://stacks.cdc.gov/view/cdc/41729/cdc_41729_DS1.pdf
• File Type:
  [PDF - 1.84 MB ]