Key Determinants of the Fungal and Bacterial Microbiomes in Homes

Details

• Alternative Title:
  Environ Res
• Personal Author:
  Kettleson, Eric M. ; Adhikari, Atin ; Vesper, Stephen ; Chatterjee, Kanistha ; Indugula, Reshmi ; Reponen, Tiina
• Description:
  Backgroundy
  
  The microbiome of the home is of great interest because of its possible impact on health. Our goal was to identify some of the factors that determine the richness, evenness and diversity of the home's fungal and bacterial microbiomes.
  
  Methods
  
  Vacuumed settled dust from homes (n=35) in Cincinnati, OH, were analyzed by pyrosequencing to determine the fungal and bacterial relative sequence occurrence. The correlation coefficients between home environmental characteristics, including age of home, Environmental Relative Moldiness Index (ERMI) values, occupant number, relative humidity and temperature, as well as pets (dog and cat) were evaluated for their influence on fungal and bacterial communities. In addition, linear discriminant analysis (LDA) was used for identifying fungal and bacterial genera and species associated with those housing determinants found to be significant.
  
  Results
  
  The fungal richness was found to be positively correlated with age of home (p=0.002), ERMI value (p=0.003), and relative humidity (p=0.015) in the home. However, fungal evenness and diversity were only correlated with the age of home (p=0.001). Diversity and evenness (not richness) of the bacterial microbiome in the homes were associated with dog ownership.
  
  Conclusions
  
  Some key determinants of the fungal and bacterial microbiome appear to be excess moisture, age of the home and dog ownership.
  
  
• Subjects:
  Air Microbiology Animals Asthma Bacteria Cats Cohort Studies DNA, Bacterial DNA, Fungal DNA, Ribosomal Spacer Dogs Environmental Microbiology Environmental Monitoring Fungi Housing Kentucky Microbiota Molecular Sequence Data Ohio Risk Factors RNA, Ribosomal, 16S Socioeconomic Factors

  More +
• Keywords:
  Bacteria Fungi Indoor Environment Microbial Diversity Microbiome
• Source:
  Environ Res. 2015; 138:130-135
• Pubmed ID:
  25707017
• Pubmed Central ID:
  PMC4385485
• Document Type:
  Journal Article
• Funding:
  R01 ES011170/ES/NIEHS NIH HHSUnited States/ ; T32 ES010957/ES/NIEHS NIH HHSUnited States/ ; T42 OH008432/OH/NIOSH CDC HHSUnited States/ ; T42OH008432-07/OH/NIOSH CDC HHSUnited States/ ; T32ES010957-11/ES/NIEHS NIH HHSUnited States/ ; ES11170/ES/NIEHS NIH HHSUnited States/ ; UL1 TR001425/TR/NCATS NIH HHSUnited States/
• Volume:
  138
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:0e6e37002841296332ffcebe1f18cdedc96c267fff00e7f8e6e1333f8905df60
• Download URL:
  https://stacks.cdc.gov/view/cdc/31238/cdc_31238_DS1.pdf
• File Type:
  [PDF - 461.21 KB ]