Key determinants of the fungal and bacterial microbiomes in homes

Details

• Personal Author:
  Adhikari A ; Coombs K ; Indugula R ; Kettleson EM ; Reponen T ; Vesper S
• Description:
  BACKGROUND: The microbiome of the home is of great interest because of its possible impact on health. Our goal was to identify some of the factors that determine the richness, evenness and diversity of the home's fungal and bacterial microbiomes. METHOD: Vacuumed settled dust from homes (n=35) in Cincinnati, Ohio, were analyzed by pyrosequencing to determine the fungal and bacterial relative sequence occurrence. The correlation coefficients between home environmental characteristics, including age of home, Environmental Relative Moldiness Index (ERMI) values, occupant number, relative humidity and temperature, as well as pets (dog and cat) were evaluated for their influence on fungal and bacterial communities. In addition, linear discriminant analysis (LDA) was used for identifying fungal and bacterial genera and species associated with those housing determinants found to be significant. RESULTS: The fungal richness was found to be positively correlated with age of home (p=0.002), ERMI value (p=0.003), and relative humidity (p=0.015) in the home. However, fungal evenness and diversity were only correlated with the age of home (p=0.001). Diversity and evenness (not richness) of the bacterial microbiome in the homes were associated with dog ownership. Linear discriminant analysis showed total of 39 putative fungal genera/species with significantly higher LDA scores in high ERMI homes and 47 genera/species with significantly higher LDA scores in homes with high relative humidity. When categorized according to the age of the home, a total of 67 fungal genera/species had LDA scores above the significance threshold. Dog ownership appeared to have the most influence on the bacterial microbiome, since a total of 130 bacterial genera/species had significantly higher LDA scores in homes with dogs. CONCLUSIONS: Some key determinants of the fungal and bacterial microbiome appear to be excess moisture, age of the home and dog ownership. [Description provided by NIOSH]
• Subjects:
  Air Pollution, Indoor Animals Dust Environment Fungi Humidity Microbiology Occupational Health Safety Temperature
• Keywords:
  Author Keywords: Indoor Environment Bacteria Dust Analysis Environmental Factors Indoor Environmental Quality Microbial Diversity Microbiome Microorganisms Molds Relative Humidity Temperature Effects

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• ISSN:
  0013-9351
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Georgia ; Kentucky ; Ohio ; OSHA Region 4 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  130-135
• Volume:
  138
• NIOSHTIC Number:
  nn:20046357
• Citation:
  Environ Res 2015 Apr; 138:130-135
• Contact Point Address:
  Eric M. Kettleson, University of Cincinnati, Department of Environmental Health, P.O. Box 670056, 3223 Eden Ave., Cincinnati, OH 45267-0056, United States
• Email:
  emkettleson@gmail.com
• Federal Fiscal Year:
  2015
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Environmental Research
• End Date:
  20260630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c034a98737275ada25279c4641da798d70ede021f1763769c11e0f055f496c9eaf3de28e0491b900374b9b1f736bb64023737cc35b6e95df14e21e89218f6cbc
• Download URL:
  https://stacks.cdc.gov/view/cdc/201442/cdc_201442_DS1.pdf
• File Type:
  [PDF - 886.68 KB ]