Manganese Accumulation in Bone Following Chronic Exposure in Rats: Steady-State Concentration and Half-life in Bone

Details

• Alternative Title:
  Toxicol Lett
• Personal Author:
  O’Neal, Stefanie L. ; Hong, Lan ; Fu, Xue ; Jiang, Wendy ; Jones, Alexander ; Nie, Linda H. ; Zheng, Wei
• Description:
  Literature data indicate that bone is a major storage organ for manganese (Mn), accounting for 43% of total body Mn. However, the kinetic nature of Mn in bone, especially the half-life (t(1/2)), remained unknown. This study was designed to understand the time-dependence of Mn distribution in rat bone after chronic oral exposure. Adult male rats received 50 mg Mn/kg (as MnCl2) by oral gavage, 5 days per week, for up to 10 weeks. Animals were sacrificed every 2 weeks during Mn administration for the uptake study, and on day 1, week 2, 4, 8, or 12 after the cessation at 6-week Mn exposure for the t(1/2) study. Mn concentrations in bone (MnBn) were determined by AAS analysis. By the end of 6-week's treatment, MnBn appeared to reach the steady state (T(ss)) level, about 2-3.2 fold higher than MnBn at day 0. Kinetic calculation revealed t(1/2)s of Mn in femur, tibia, and humerus bone of 77 (r=0.978), 263 (r=0.988), and 429 (r=0.994) days, respectively; the average t(1/2) in rat skeleton was about 143 days, equivalent to 8.5 years in human bone. Moreover, MnBn were correlated with Mn levels in striatum, hippocampus, and CSF. These data support MnBn to be a useful biomarker of Mn exposure.
• Subjects:
  Algorithms Animals Body Weight Bone And Bones Brain Central Nervous System Half-Life Kinetics Magnesium Chloride Male Manganese Metals Muscle, Skeletal Organ Size Pharmacokinetics Rats Rats, Sprague-Dawley Spectrophotometry, Atomic Tissue Distribution

  More +
• Keywords:
  Biomarker Bone Disposition Half-life
• Source:
  Toxicol Lett. 2014; 229(1):93-100
• Pubmed ID:
  24930841
• Pubmed Central ID:
  PMC4126163
• Document Type:
  Journal Article
• Funding:
  R01 ES008146/ES/NIEHS NIH HHSUnited States/ ; R21 OH010044/OH/NIOSH CDC HHSUnited States/ ; R21-OH010044/OH/NIOSH CDC HHSUnited States/ ; R01-ES008146/ES/NIEHS NIH HHSUnited States/
• Volume:
  229
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:04a3de5a8e2033491279099d1771c7d8dbd07e45269a539ef164a9536020dd36
• Download URL:
  https://stacks.cdc.gov/view/cdc/30071/cdc_30071_DS1.pdf
• File Type:
  [PDF - 877.43 KB ]