Magnetic Barcode Assay for Genetic Detection of Pathogens

Details

• Alternative Title:
  Nat Commun
• Personal Author:
  Liong, Monty ; Hoang, Anh N. ; Chung, Jaehoon ; Gural, Nil ; Ford, Christopher B. ; Min, Changwook ; Shah, Rupal R. ; Ahmad, Rushdy ; Fernandez-Suarez, Marta ; Fortune, Sarah M. ; Toner, Mehmet ; Lee, Hakho ; Weissleder, Ralph
• Description:
  The task of rapidly identifying patients infected with Mycobacterium tuberculosis in resource-constrained environments remains a challenge. A sensitive and robust platform that does not require bacterial isolation or culture is critical in making informed diagnostic and therapeutic decisions. Here we introduce a platform for the detection of nucleic acids based on a magnetic barcoding strategy. PCR-amplified mycobacterial genes are sequence-specifically captured on microspheres, labelled by magnetic nanoprobes and detected by nuclear magnetic resonance. All components are integrated into a single, small fluidic cartridge for streamlined on-chip operation. We use this platform to detect M. tuberculosis and identify drug-resistance strains from mechanically processed sputum samples within 2.5 h. The specificity of the assay is confirmed by detecting a panel of clinically relevant non-M. tuberculosis bacteria, and the clinical utility is demonstrated by the measurements in M. tuberculosis-positive patient specimens. Combined with portable systems, the magnetic barcode assay holds promise to become a sensitive, high-throughput and low-cost platform for point-of-care diagnostics.
• Subjects:
  Article Base Sequence DNA, Bacterial DNA Barcoding, Taxonomic Genome, Bacterial Humans Magnetics Molecular Sequence Data Mutation Mycobacterium Tuberculosis Polymerase Chain Reaction Polymorphism, Single Nucleotide Sensitivity And Specificity

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• Source:
  Nat Commun. 4:1752
• Pubmed ID:
  23612293
• Pubmed Central ID:
  PMC3635151
• Document Type:
  Journal Article
• Funding:
  R01-HL113156/HL/NHLBI NIH HHS/United States ; DP2 0D001378/DP/NCCDPHP CDC HHS/United States ; T32 CA079443/CA/NCI NIH HHS/United States ; U19 AI076217/AI/NIAID NIH HHS/United States ; HHSN268201000044C/HL/NHLBI NIH HHS/United States ; R01 EB010011/EB/NIBIB NIH HHS/United States ; R01-EB010011/EB/NIBIB NIH HHS/United States ; T32 AI007638/AI/NIAID NIH HHS/United States ; DP2 OD001378/OD/NIH HHS/United States ; T32CA79443/CA/NCI NIH HHS/United States ; R01-EB00462605A1/EB/NIBIB NIH HHS/United States ; R01 EB004626/EB/NIBIB NIH HHS/United States ; HHSN268201000044C/PHS HHS/United States ; R01 HL113156/HL/NHLBI NIH HHS/United States ; P41 EB002503/EB/NIBIB NIH HHS/United States
• Volume:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:ef7a9882e2c2d26cfa5b1ed673a9271565b4ddf0fc6f6a94d663d39c189ac407
• Download URL:
  https://stacks.cdc.gov/view/cdc/29651/cdc_29651_DS1.pdf
• File Type:
  [PDF - 1.56 MB ]