Metabolic Profiling of Night Shift Work – the HORMONIT Study

Details

• Personal Author:
  Baker MG ; Castaño-Vinyals G ; Espinosa A ; Harding BN ; Kogevinas M ; Middleton B ; Navarrete JM ; Papantoniou K ; Skene DJ ; Such P ; Torrejón A
• Description:
  Mechanistic studies are needed to understand how rotating shift work perturbs metabolic processing. We collected plasma samples (n = 196) from 49 males, rotating car factory shift workers at the beginning and end of a night-shift (22:00-06:00 h) and day-shift (06:00 h-14:00 h). Samples underwent targeted LC-MS/MS metabolomics and concentrations of 130 metabolites were log2-transformed and pareto-scaled. An elastic net selected the most influential metabolites for linear mixed models examining within-person variation in metabolite levels at night-shift end (06:00 h) compared to day-shift start (06:00 h). Quantitative enrichment analysis explored differentially enriched biological pathways between sample time points. We included 20 metabolites (amino acids, biogenic amines, acylcarnitines, glycerophospholipids) in mixed models. Night-shift was associated with changes in concentrations of arginine (geometric mean ratio [GMR] 2.30, 95%CI 1.25, 4.23), glutamine (GMR 2.22, 95%CI 1.53, 3.24), kynurenine (GMR 3.22, 95%CI 1.05, 9.87), lysoPC18:2 (GMR 1.86, 95%CI 1.11, 3.11), lysoPC20:3 (GMR 2.48, 95%CI 1.05, 5.83), PCaa34:2 (GMR 2.27, 95%CI 1.16, 4.44), and PCae38:5 (GMR 1.66, 95%CI 1.02, 2.68). Tryptophan metabolism, glutathione metabolism, alanine metabolism, glycine and serine metabolism, and urea cycle were pathways differing between shifts. Night shift work was associated with changes in metabolites and the perturbation of metabolic and biochemical pathways related to a variety of health outcomes. [Description provided by NIOSH]
• Subjects:
  Circadian Rhythm Energy Metabolism Occupational Health Safety Shift Work Schedule
• Keywords:
  Author Keywords: Circadian Biochemical Markers Biological Monitoring Circadian Rhythms Metabolic Effects Metabolism Metabolomics Occupational Health Rotating Shift Work Shift Work Shift Worker Shift Workers Work Schedules

  More +
• ISSN:
  0742-0528
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  39
• Issue:
  11
• NIOSHTIC Number:
  nn:20066911
• Citation:
  Chronobiol Int 2022 Nov; 39(11):1508-1516
• Contact Point Address:
  Marissa G. Baker, Department of Environmental & Occupational Health Sciences, University of Washington, Seattle, Washington, USA
• Email:
  bakermg@uw.edu
• Federal Fiscal Year:
  2023
• Performing Organization:
  University of Washington
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Chronobiology International
• End Date:
  20150630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:59496ffac5f2e0f8612fc8fc5612e95332ec183fdea5cf548134f287f3cfb9bcbafe00fa20ef4ff4c28820374d68f7e37689c775ee5063cf4cf5a1d9bdbec196
• Download URL:
  https://stacks.cdc.gov/view/cdc/231204/cdc_231204_DS1.pdf
• File Type:
  [PDF - 814.24 KB ]