Mineral Surface-Specific Differences in the Adsorption and Enzymatic Removal of Surfactant and Their Correlation with Cytotoxicity

Details

• Personal Author:
  Hill CA ; Keane MJ ; Mike PS ; Vallyathan V ; Wallace WE
• Description:
  Both respirable quartz and kaolin dusts are cytotoxic to pulmonary macrophage in vitro. This native dust cytotoxic potential does not provide a basis for distinguishing the comparative pulmonary disease inducing potentials of the dusts. When the mineral dusts are incubated in dipalmitoyl glycerophosphorylcholine (lecithin) dispersed in physiologic saline, a model of initial dust interaction within the pulmonary alveolar hypophase, cytotoxicity of both dusts are suppressed by surface adsorption of the surfactant. Lecithin coated dusts were subsequently incubated with phospholipase A2 enzyme as a model for a phagolysosomal enzymatic digestion process following phagocytosis. The enzyme activity for digestion of adsorbed lecithin was quantitated by chemical phosphate assay for remaining lecithin and digestion products, and by erythrocyte hemolysis assay for restoration of dust lytic potential as functions of applied enzyme activity and digestion time. Data indicate quartz surface lytic potential is restored within two to three days at applied enzymatic activities under which a significantly greater lytic suppression property is retained by kaolin. Potential hydrogen bonding and electrostatic interactions of surfactant and mineral surface functional groups are compared as a basis for mineral-specific differences in the enzyme digestion kinetics of adsorbed surfactant. This mineral-surface specific difference in rates of restoration of dust cytotoxic potential and existing data on pulmonary clearance rates are reviewed in the context of a mechanism distinguishing the relative pathogenic potential of quartz in comparison with aluminosilicate, respirable dusts. Description provided by NIOSH
• Subjects:
  • Cytotoxins
  • Dust
  • Enzymes
  • Occupational Health
  • Quartz
  • Safety
  • Silicon Dioxide
  • Surface-Active Agents
• Keywords:
  • Surfactants
  • Cytotoxic effects
  • Enzyme activity
  • Adsorption
  • Respirable dust
  • Mineral dusts
  • Quartz dust
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  16
• NIOSHTIC Number:
  nn:20059123
• Citation:
  Fourth International Workshop on Effects of Mineral Dusts on Cells, September 21-23, 1988, Orford, Quebec, Canada. Brussels, Belgium: N.A.T.O. (North Atlantic Treaty Organization), 1988 Sep; :16
• CAS Registry Number:
  • Kaolin (CAS RN 1332-58-7)
  • Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  1988
• Peer Reviewed:
  False
• Source Full Name:
  Fourth International Workshop on Effects of Mineral Dusts on Cells, September 21-23, 1988, Orford, Quebec, Canada
• Download URL:
  https://stacks.cdc.gov/view/cdc/223436/cdc_223436_DS1.pdf
• File Type:
  [PDF - 874.05 KB]
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ff994dbfbc6debfaa5a3df16ed8980043f7a60220fbda2768ded206e16bbac40c2e0bf4444d4a970857514ca7a4440686328e41bfcc6f3d7df2cae86474ce522