The enzymatic removal of a surfactant coating from quartz and kaolin by P388D1 cells

Details

• Personal Author:
  Hill CA ; Keane MJ ; Mike PS ; Wallace WE
• Description:
  A study was conducted examining the ability of macrophages to remove a dipalmitoyl-phosphatidylcholine(DPL) coating from the surface of particles. The P388D1 macrophage cell line was incubated with DPL coated quartz (14808607) and kaolin (1332587) particles and the removal of DPL was examined for up to 9 days. P388D1 digested DPL from the surface of both quartz and kaolin particles at a rate that was independent of the dust concentration and of the amount of coated dust within the cell at lower concentration levels. The rate of digestion decreased slightly at the highest concentration of dust used. These results were suggestive of extracellular phospholipase activity. Characterization of the extracellular phospholipase activity demonstrated that it was inhibited by increasing temperatures, chlorphentermine, imipramine, chloroquine, ethylenediaminetetraacetic-acid, decreasing pH, and pretreatment with proteinase-K or trypsin, and unaffected by freezing and thawing. These characteristics were consistent with an extralysosomal phospholipase active at pH7 and dependent upon the presence of divalent metal ions. The rate of DPL removal by the extracellular phospholipase from kaolin was slower than the rate of removal from quartz. [Description provided by NIOSH]
• Subjects:
  Dust Enzymes Lung Occupational Health Quartz Respiratory System Safety Silicon Dioxide Surface-Active Agents
• Keywords:
  Author Keywords: Dipalmitoyl Phosphatidyl Choline Dose-response Enzyme-activity In-vitro-study Kaolin Metallic-ions NIOSH-Author P388D1 Pulmonary-system Quartz Quartz-dust Surfactant Surfactants

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• ISSN:
  0742-2091
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  119-128
• Volume:
  11
• Issue:
  2
• NIOSHTIC Number:
  nn:00232504
• Citation:
  Cell Biol Toxicol 1995 Apr; 11(2):119-128
• CAS Registry Number:
  Kaolin (CAS RN 1332-58-7) ; Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  1995
• Peer Reviewed:
  True
• Source Full Name:
  Cell Biology and Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ee45108e511abe77d54fb26e6b8473de489e5365e5f35ff9dca50a1442e2dd31b1724d1df752bf298991d550b69d98c176b0328f3e95056270f323fcac1ad82d
• Download URL:
  https://stacks.cdc.gov/view/cdc/205724/cdc_205724_DS1.pdf
• File Type:
  [PDF - 849.11 KB ]