Joint Toxicity of a Multi-Heavy Metal Mixture and Chemoprevention in Sprague Dawley Rats

Details

• Personal Author:
  Ding M ; Hua Q ; Li Z ; Song X ; Tang C ; Tang Y ; Wang L ; Wang Y ; Zhao J ; Zou B
• Description:
  To explore the joint toxicity and bio-accumulation of multi-heavy metals and potential chemoprevention strategies, Male Sprague Dawley (SD) rats (n = 30) were treated orally once a week for six months with 500mg/kg.bw of eight heavy metals which were commonly identified in aquatic products in the Ningbo area including chromium, manganese, nickel, copper, zinc, cadmium, mercury, and lead. At the same time, 200mg/kg.bw of epigallocatechin-3-gallate (EGCG), trisodium citrate dihydrate (TCD) or glutathione (GSH) were administered to evaluate their antagonistic effects against adverse effects of multi-heavy metal mixture. The Morris water maze test was used to evaluate spatial learning and memory in the treated rats. Then the rats were anesthetized by pentobarbital sodium (40 mg/kg.bw) to obtain blood samples for biochemical analysis and organs (heart, liver, spleen, lungs, kidneys, brain, testis) to be conducted for biopsy and organ coefficients. Inductively coupled plasma mass spectrometer (ICP-MS) was used to analyze the concentrations of heavy metals. Results indicated that six months of exposure to a multi-heavy metal mixture under this experimental dosage resulted in accumulation in organs and adverse effects on the blood, reproductive system, and liver function. EGCG, TCD or GSH all showed certain chemoprevention effects against the joint toxicity induced by the multi-heavy metal mixture and indicated alleviation and the potential mechanism that also included the promotion of excretion of metals to which animals were exposed. [Description provided by NIOSH]
• Subjects:
  Animals Biochemistry Blood Cadmium Chromium Copper Environmental Exposure Hazardous Substances Hematologic Tests Laboratories Lead Manganese Mercury Metals, Heavy Neoplasms Nickel Occupational Health Risk Assessment Risk Factors Safety Urogenital System

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• Keywords:
  Accumulation Antagonism Author Keywords: Multi-heavy Metal Mixture Biochemical Analysis Blood Samples Chemoprevention Chemotherapy Exposure Levels Health Effects Heavy Metals Joint Toxicity Laboratory Animals Liver Function Mass Spectrometry Reproductive System Risk Factors Sprague Dawley Rats Toxins Zinc

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• ISSN:
  1660-4601
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  17
• Issue:
  4
• NIOSHTIC Number:
  nn:20058897
• Citation:
  Int J Environ Res Public Health 2020 Feb; 17(4):1451
• Contact Point Address:
  Jinshun Zhao, Department of Preventative Medicine, Zhejiang Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo 315211, Zhejiang, China
• Email:
  zhaojinshun@nbu.edu.cn
• CAS Registry Number:
  Cadmium (CAS RN 7440-43-9) ; Chromium (CAS RN 7440-47-3) ; Copper (CAS RN 7440-50-8) ; Glutathione (CAS RN 70-18-8) ; Lead (CAS RN 7439-92-1) ; Manganese (CAS RN 7439-96-5) ; Mercury (CAS RN 7439-97-6) ; Nickel (CAS RN 7440-02-0) ; Sodium citrate dihydrate (CAS RN 6132-04-3) ; Zinc (CAS RN 7440-66-6)
• Federal Fiscal Year:
  2020
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  International Journal of Environmental Research and Public Health
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fdae25d78834a5ad1301239ee29f0f28fe8c68125409465e0e4cacf426deeff3ae78e9a638c7554be427eb38a2619d3784d01f7c97b23f0c0e244b2cca09e977
• Download URL:
  https://stacks.cdc.gov/view/cdc/223259/cdc_223259_DS1.pdf
• File Type:
  [PDF - 2.55 MB ]