Antigen Profiling of Field Metalworking Fluids

Details

• Personal Author:
  Chandra H ; Lam YW ; Yadav JS
• Description:
  Machine workers repeatedly exposed to the in-use contaminated metal working fluid (MWF) aerosols are at increased risk of developing occupational respiratory conditions including hypersensitivity pneumonitis (HP), asthma, or other respiratory symptoms over a period of time. Machinists' HP is a cell-mediated immune disease associated with repeated exposure to the mycobacterial species Mycobacterium immunogenum frequently isolated from these industrial fluids. However, the specific T-cell antigens/epitopes responsible for causing the MWF exposure-associated HP in the exposed machinists are not yet defined. In this study, we hypothesize that the prevailing field conditions in MWF can lead to induction and release of specific antigens from mycobacteria that are critical for HP etiology. Overall aim is to identify mycobacterium-specific critical HP-inciting antigens directly from the mycobacteriac ontaminated in-use MWF samples from the automotive industries. We propose to accomplish this using cutting edge immuno-proteomics approach coupled with immunoinformatic tools. The in-silico identified candidate antigenic peptides will be validated for functional T-cell response in an in-vitro T cell assay. These identified antigens could provide a key to understanding the etiological aspects of the disease development and facilitate development of immunodiagnosis and intervention strategies for HP patients. [Description provided by NIOSH]
• Subjects:
  Antigens Immune System Lung Diseases Manufacturing And Industrial Facilities Occupational Health Respiratory Tract Diseases Safety
• Keywords:
  Cell Biology Cellular Effects Hypersensitivity Pneumonitis Immune Reaction Immunodiagnosis Lung Disease Machinists Metalworking Fluids MWFs Mycobacterium
• Publisher:
  University of Cincinnati
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 1 ; OSHA Region 5 ; Vermont
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1
• NIOSHTIC Number:
  nn:20052391
• Citation:
  16th Annual Pilot Research Project Symposium, University of Cincinnati Education and Research Center, October 8-9, 2015, Cincinnati, Ohio. Cincinnati, OH: University of Cincinnati, 2015 Oct; :1
• Email:
  chandrhh@ucmail.uc.edu
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  16th Annual Pilot Research Project Symposium, University of Cincinnati Education and Research Center, October 8-9, 2015, Cincinnati, Ohio
• End Date:
  20170731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:efef4793453ac5c9ca04d16004657f0163eb4f92b4fb32c48b4df90e4cccd075d5e10da497f2e348112b5c7be73f133d27c3b2f7c59a41cae8a778e1dc4b1d35
• Download URL:
  https://stacks.cdc.gov/view/cdc/211922/cdc_211922_DS1.pdf
• File Type:
  [PDF - 141.45 KB ]