Novel Antigens of Mycobacterium immunogenum for Serodiagnosis of Hypersensitivity Pneumonitis

Details

• Personal Author:
  Chandra H ; Yadav JS
• Description:
  Mycobacterium immunogenum of the M. abscessus M. chelonae complex of non-tuberculous mycobacteria is the newly-recognized etiological agent of occupational hypersensitivity pneumonitis (HP) in machinists. Diagnosis and pathogenesis mechanisms of this immune-mediated disease are poorly understood. The aim of this study was to identify critical antigens of M. immunogenum for serological diagnosis of HP patients. Our initial immunoproteomic study had identified 33 immuno-reactive proteins from different fractions of the cell. In this study, recombinant forms of three cell wall-associated antigens (arbitrarily designated as antigen A through C) and two secretory antigens (D & E) expressed and purified as His tagged proteins were evaluated using blood sera from HP patients and healthy subjects (n=11). The antigens tested showed high sensitivity (87.5%) and specificity (100%) with 95% confidence interval (0.505-0.995) for optimal threshold, except antigen D which showed 50% (0.217-0.783) sensitivity (95% CI) and 100% (0.380-1.000) specificity (95% CI). Comparison in terms of the receiver operating characteristics (ROC) curve showed highly significant AUC values (p value < 0.0001 for AgA through AgC and <0.05 for Ag D) considering the 0.5 cut off value; the AUC values followed the order Ag A (0.958) = Ag C (0.958) >Ag E (0.917) > Ag B (0.875) >Ag D (0.750). Taken together, this study provides a panel of critical antigens with high sensitivity and specificity for the needed discrimination between HP patients and healthy subjects and paves the way for developing future immunodiagnostic tools for occupational HP. [Description provided by NIOSH]
• Subjects:
  Antigens Immune System Lung Diseases Manufacturing And Industrial Facilities Occupational Health Respiratory Tract Diseases Safety
• Keywords:
  Author Keywords: Mycobacterium Immunogenum Cell Biology Cellular Effects Exposure Assessment Hypersensitivity Pneumonitis Immune Reaction Immunodiagnosis Lung Disease Machinists Metalworking Fluids MWFs Mycobacterium

  More +
• Publisher:
  American Society for Microbiology
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20052390
• Citation:
  ASM 2014: Proceedings of the 114th American Society for Microbiology General Meeting, May 17-20, 2014, Boston, Massachusetts. Washington, DC: American Society for Microbiology, 2014 May; :937
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  ASM 2014: Proceedings of the 114th American Society for Microbiology General Meeting, May 17-20, 2014, Boston, Massachusetts
• End Date:
  20170731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7351a30f1dc9dc416157d8fa4bb03bec925797f0dc2ccdf735edcadd1bc99331f49286a814a0c6e39bd32cfcbe0e0efb724f8c3cd4af7e8782142f3636136f44
• Download URL:
  https://stacks.cdc.gov/view/cdc/211921/cdc_211921_DS1.pdf
• File Type:
  [PDF - 98.08 KB ]