High Performance Liquid Chromatography Identification, Quantification, and Fractionation of a Suspect Allergen, 4-Chloro-3-Methylphenol, in an LLNA Positive Metalworking Fluid

Details

• Personal Author:
  Brown KK ; Robinson K
• Description:
  A metalworking fluid (MWF) was obtained that produced an allergic reaction in the local lymph node assay (LLNA) with an EC3 = 4% (estimated concentration needed to provoke a three-fold allergy response). The biocide, 4-chloro-3-methylphenol, was detected in the MWF as a chromatographic peak matching the retention time of an external standard. The two mobile phase solutions were concentrated back into a MWF and a 4-chloro-3- methylphenol fraction. The semi-preparative HPLC method resulted in reproducible chromatograms for the seventeen fractionations of MWF. One run was sufficient to fractionate a metalworking fluid for component analysis. The two recovered fractions, the 4-chloro-3-methylphenol and the remaining MWF, were reconstituted by nitrogen evaporation to a volume equal to its original concentration. This method can be used to fractionate MW for COC-GC-MS analysis that requires trace enrichment and LLNA that requires 1000 mg of sample. [Description provided by NIOSH]
• Subjects:
  Biological Assay Chemistry Chromatography Hypersensitivity Laboratories Lubricants Lymphatic System Occupational Health Phenol Safety Spectrum Analysis Toxicology

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• Keywords:
  Allergens Allergic Reactions Analytical Instruments Analytical Processes Bioassays Biocides Chemical Analysis Chromatographic Analysis Gas Liquid Chromatography Laboratory Techniques Liquid Chromatography Lymph Nodes Metalworking Fluids Methyl Compounds Phenols Spectrographic Analysis

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• ISBN:
  9781604233261
• Publisher:
  Curran Associates, Inc.
• Document Type:
  Text
• Genre:
  Proceedings
• Place as Subject:
  District of Columbia ; Ohio ; OSHA Region 3 ; OSHA Region 5 ; Pennsylvania
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  68-88
• Volume:
  1
• NIOSHTIC Number:
  nn:20051544
• Citation:
  62nd Annual Meeting of the Society of Tribologists and Lubrication Engineers 2007, May 6-10, 2007, Philadelphia, Pennsylvania. Red Hook, NY: Curran Associates, Inc., 2007 Sep; 1:68-88
• Contact Point Address:
  Kenneth K. Brown, Research Chemist, Division of Applied Research and Technology, National Institute for Occupational Safety and Health, 4676 Columbia Parkway MS-R7, Cincinnati, Ohio 45226
• Email:
  krb1@cdc.gov
• CAS Registry Number:
  4-Chloro-3-methylphenol (CAS RN 59-50-7)
• Federal Fiscal Year:
  2007
• Peer Reviewed:
  False
• Source Full Name:
  62nd Annual Meeting of the Society of Tribologists and Lubrication Engineers 2007, May 6-10, 2007, Philadelphia, Pennsylvania
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c60960350c98fe04abcd71340f063756bab7c0155752e3f8181ab2c787b67c4985c71d8ad7cb3a94f50626f62659e3b76d595ac578ea0e54488a0bd2d28891a2
• Download URL:
  https://stacks.cdc.gov/view/cdc/211444/cdc_211444_DS1.pdf
• File Type:
  [PDF - 833.14 KB ]