High-Performance Liquid Chromatography Identification, Quantification, and Fractionation of a Suspect Allergen, 4-Chloro-3-Methylphenol, in an LLNA-Positive Metalworking Fluid

Details

• Personal Author:
  Brown KK ; Robinson K
• Description:
  A metalworking fluid (MWF) was obtained that produced an allergic reaction in the local lymph node assay (LLNA) with an EC3 = 4%, the EC3 being the estimated concentration needed to provoke a 3-fold allergy response. High-performance liquid chromatography (HPLC) was used to separate, identify, and isolate the suspected allergen. The biocide, 4-chloro-3-methylphenol, was detected in the MWF as a chromatographic peak matching the retention time of an external standard. The technique of standard addition was used to quantify and confirm the presence of 4-chloro-3-methylphenol at about 1% (w/w). Preparative HPLC was used to fractionate 1 gram of MWF separating the 4-chloro-3-methylphenol fraction from the remaining MWF. The two mobile-phase solutions were concentrated back into an MWF and a 4-chloro-3-methylphenol fraction. The original MWF and the reconstituted 4-chloro-3-methylphenol and MWF fractions were also analyzed by gas chromatography-mass spectrometry to confirm the isolation of the biocide. [Description provided by NIOSH]
• Subjects:
  Biological Assay Chemistry Chromatography Chromatography, Gas Hypersensitivity Lubricants Metals Occupational Health Safety Spectrum Analysis Toxicology

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• Keywords:
  Allergens Allergic-reactions Bioassays Biocides Chemical-analysis Chromatographic-analysis Gas-liquid-chromatography Liquid-chromatography Lymph-nodes Metalworking-fluids Spectrographic-analysis

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• ISSN:
  1040-2004
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  District of Columbia ; Ohio ; OSHA Region 3 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  101-106
• Volume:
  51
• Issue:
  1
• NIOSHTIC Number:
  nn:20034610
• Citation:
  Tribol Trans 2008 Jan; 51(1):101-106
• Contact Point Address:
  National Institute for Occupational Safety and Health, Division of Applied Research and Technology, 4676 Columbia Parkway, Cincinnati, OH 45226
• CAS Registry Number:
  4-Chloro-3-methylphenol (CAS RN 59-50-7)
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Tribology Transactions
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:35196d9de9fe87016ca57b0fce7bd1d1efa8b5b5356fc62ad74599d6cdb654fbcfe0a840a9ccaa8f97a0a6b4d831bab7e4a49959faf4e248bd1bdc459cd26879
• Download URL:
  https://stacks.cdc.gov/view/cdc/186757/cdc_186757_DS1.pdf
• File Type:
  [PDF - 2.81 MB ]