High-performance liquid chromatography identification, quantification, and fractionation of a suspect allergen, 4-chloro-3-methylphenol, in an LLNA-positive metalworking fluid

Details

• Personal Author:
  Brown KK ; Robinson K
• Description:
  A metalworking fluid (MWF) was obtained that produced an allergic reaction in the local lymph node assay (LLNA) with an EC3 = 4%, the EC3 being the estimated concentration needed to provoke a 3-fold allergy response. High-performance liquid chromatography (HPLC) was used to separate, identify, and isolate the suspected allergen. The biocide, 4-chloro-3-methylphenol, was detected in the MWF as a chromatographic peak matching the retention time of an external standard. The technique of standard addition was used to quantify and confirm the presence of 4-chloro-3-methylphenol at about 1 % (w/w). Preparative HPLC was used to fractionate 1 gram of MWF separating the 4-chloro-3-methylphenol fraction from the remaining MWF. The two mobile-phase solutions were concentrated back into an MWF and a 4-chloro-3-methylphenol fraction. The original MWF and the reconstituted 4-chloro-3-methylphenol and MWF fractions were also analyzed by gas chromatography-mass spectrometry to confirm the isolation of the biocide. [Description provided by NIOSH]
• Subjects:
  Biological Assay Chemistry Chromatography Chromatography, Gas Hypersensitivity Lubricants Maintenance Metals Occupational Health Safety Spectrum Analysis Toxicology

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• Keywords:
  Allergens Allergic-reactions Author Keywords: Biocides Bioassays Biocides Chemical-analysis Chemical Contamination Chromatographic-analysis Cleanliness Gas-liquid-chromatography Gas Chromatography Hygiene Liquid-chromatography Liquid Chromatography Lubricant Microbial Decomposition Lymph-nodes Metalworking-fluids Metalworking Fluids Safety: Toxicology Spectrographic-analysis Spectroscopy

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• ISSN:
  1545-858X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  District of Columbia ; Ohio ; OSHA Region 3 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  71
• Issue:
  11
• NIOSHTIC Number:
  nn:20047097
• Citation:
  Tribol Lubr Technol 2015 Nov; 71(11):97-101,103,105
• Contact Point Address:
  National Institute for Occupational Safety and Health, Division of Applied Research and Technology, 4676 Columbia Parkway, Cincinnati, OH 45226
• CAS Registry Number:
  4-Chloro-3-methylphenol (CAS RN 59-50-7)
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  All Sectors
• Peer Reviewed:
  True
• Source Full Name:
  Tribology & Lubrication Technology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1e9b1523c6d0a487b6061329cbdf9fb2fc9b8d915fbd312d14a8fabc5fa699a732b69a2e7ecd90cb827d2b5ebc39d553af346cf643a41c1462507ac07ad6593f
• Download URL:
  https://stacks.cdc.gov/view/cdc/202294/cdc_202294_DS1.pdf
• File Type:
  [PDF - 836.42 KB ]