High-Fat Western Diet Alters Crystalline Silica-Induced Airway Epithelium Ion Transport but Not Airway Smooth Muscle Reactivity

Details

• Personal Author:
  Fedan, Jeffery S. ; Kashon ML ; McKinney W ; Thompson JA
• Description:
  Objectives: Silicosis is an irreversible occupational lung disease resulting from crystalline silica inhalation. Previously, we discovered that Western diet (HFWD)-consumption increases susceptibility to silica-induced pulmonary inflammation and fibrosis. This study investigated the potential of HFWD to alter silica-induced effects on airway epithelial ion transport and smooth muscle reactivity. Methods: Six-week-old male F344 rats were fed a HFWD or standard rat chow (STD) and exposed to silica (Min-U-Sil 5®, 15 mg/m3, 6 h/day, 5 days/week, for 39 d) or filtered air. Experimental endpoints were measured at 0, 4, and 8 weeks post-exposure. Transepithelial potential difference (Vt), short-circuit current (ISC) and transepithelial resistance (Rt) were measured in tracheal segments and ion transport inhibitors [amiloride, Na+ channel blocker; NPPB; Cl¯ channel blocker; ouabain, Na+, K+-pump blocker] identified changes in ion transport pathways. Changes in airway smooth muscle reactivity to methacholine (MCh) were investigated in the isolated perfused trachea preparation. Results: Silica reduced basal ISC at 4 weeks and HFWD reduced the ISC response to amiloride at 0 week compared to air control. HFWD + silica exposure induced changes in ion transport 0 and 4 weeks after treatment compared to silica or HFWD treatments alone. No effects on airway smooth muscle reactivity to MCh were observed. [Description provided by NIOSH]
• Subjects:
  Animals Diet Musculoskeletal System Occupational Health Respiratory System Safety Silicon Dioxide
• Keywords:
  Animal Studies Author Keywords: Airway Hyperreactivity Crystalline Silica Dietary Effects Epithelium Inhalation Studies Ion Transport Muscle Function Obesity Silica Silicosis Western Diet

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• ISSN:
  1756-0500
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  13
• Volume:
  17
• NIOSHTIC Number:
  nn:20069088
• Citation:
  BMC Res Notes 2024 Jan; 17:13
• Contact Point Address:
  Janet A. Thompson, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV, 26505, USA
• Email:
  jsd7@cdc.gov
• CAS Registry Number:
  Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  2024
• NORA Priority Area:
  Construction ; Mining
• Peer Reviewed:
  True
• Source Full Name:
  BMC Research Notes
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fd9d46c1eba7d62a385ea3d6f235b0cf323b0928e8c1fe17ff569a2a17a53af0ab30a69ae2b043afc56995ab1f8758558fe906434642ca672e650bcb92f4b2b3
• Download URL:
  https://stacks.cdc.gov/view/cdc/207634/cdc_207634_DS1.pdf
• File Type:
  [PDF - 1.87 MB ]