High-Fat Western Diet Alters Silica-Induced Airway Epithelium Ion Exchange but Not Airway Smooth Muscle Reactivity (Dataset)

Details

• Corporate Authors:
  National Institute for Occupational Safety and Health
• Description:
  Introduction: Silicosis is an irreversible occupational lung disease resulting from crystalline silica exposure. Previously, we discovered that Western diet (HFWD)-consumption increases susceptibility to silica-induced pulmonary inflammation and fibrosis. This study investigates the potential of HFWD to alter silica-induced effects on airway epithelial ion transport and smooth muscle reactivity. General description of data collection methods: This was a laboratory-based investigation that utilized a rat model to study the effects of a high-fat Western diet on silica-induced changes in airway epithelial ion transport and airway smooth muscle reactivity. Six-week-old male F344 rats were fed a HFWD or standard rat chow (STD) and exposed to silica (Min-U-Sil 5®, 15 mg/m3, 6 h/d, 5 d/wk, for 39 d) or filtered air. Experimental endpoints were measured at 0, 4, and 8 wk post-exposure. Transepithelial potential difference (Vt), short-circuit current (ISC) and transepithelial resistance (Rt) were measured in tracheal segments and ion transport inhibitors [amiloride, Na+ channel blocker; NPPB; Cl- channel blocker; ouabain, Na+, K+-pump blocker] identified changes in ion transport pathways. Changes in airway smooth muscle reactivity to methacholine (MCh) were investigated in the isolated perfused trachea. [Description provided by NIOSH]
• Subjects:
  Animals Diet Musculoskeletal System Occupational Health Respiratory System Safety Silicon Dioxide
• Keywords:
  Animal Studies Dietary Effects Inhalation Studies Ion Transport Muscle Function Silica Silicosis
• DOI:
  https://doi.org/10.26616/NIOSHRD-1068-2023-0
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Dataset
• Genre:
  Research Dataset
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20067574
• Citation:
  Morgantown, WV: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Research Dataset RD-1068-2023-0, 2024 Jan; :dataset
• Contact Point Address:
  Pathology and Physiology Branch, HELD, NIOSH. Tel: 304.285.6056
• CAS Registry Number:
  Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  2024
• NORA Priority Area:
  Construction ; Mining
• Peer Reviewed:
  False
• Source Full Name:
  High-fat Western diet alters silica-induced airway epithelium ion exchange but not airway smooth muscle reactivity
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5ba6a1649488c45a3d7d4ee139d5e1962d416ae49726747aa05b7fd4fe79e9c3d564dd438ee43e918304e2d21dd55186ab946fd4e33e039a595007665c81df64
• Download URL:
  https://stacks.cdc.gov/view/cdc/231500/cdc_231500_DS1.pdf
• File Type:
  [PDF - 129.14 KB ]