Identification of metal oxide nanoparticles in histological samples by enhanced darkfield microscopy and hyperspectral mapping

Details

• Personal Author:
  Brenner SA ; Neu-Baker NM ; Roth GA ; Sosa Peña MdP ; Tahiliani S
• Description:
  Nanomaterials are increasingly prevalent throughout industry, manufacturing, and biomedical research. The need for tools and techniques that aid in the identification, localization, and characterization of nanoscale materials in biological samples is on the rise. Currently available methods, such as electron microscopy, tend to be resource-intensive, making their use prohibitive for much of the research community. Enhanced darkfield microscopy complemented with a hyperspectral imaging system may provide a solution to this bottleneck by enabling rapid and less expensive characterization of nanoparticles in histological samples. This method allows for high-contrast nanoscale imaging as well as nanomaterial identification. For this technique, histological tissue samples are prepared as they would be for light-based microscopy. First, positive control samples are analyzed to generate the reference spectra that will enable the detection of a material of interest in the sample. Negative controls without the material of interest are also analyzed in order to improve specificity (reduce false positives). Samples can then be imaged and analyzed using methods and software for hyperspectral microscopy or matched against these reference spectra in order to provide maps of the location of materials of interest in a sample. The technique is particularly well-suited for materials with highly unique reflectance spectra, such as noble metals, but is also applicable to other materials, such as semi-metallic oxides. This technique provides information that is difficult to acquire from histological samples without the use of electron microscopy techniques, which may provide higher sensitivity and resolution, but are vastly more resource-intensive and time-consuming than light microscopy. Description provided by NIOSH
• Subjects:
  • Histology
  • Microbiology
  • Occupational Health
  • Safety
• Keywords:
  • Nanotechnology
  • Nanomaterials
  • Nanoscale
  • Biological materials
  • Sampling
  • Sampling equipment
  • Sampling methods
  • Medical research
  • Microscopic analysis
  • Microscopy
  More +
• ISSN:
  1940-087X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Issue:
  106
• NIOSHTIC Number:
  nn:20047949
• Citation:
  J Vis Exp 2015 Dec; (106):e53315
• Contact Point Address:
  Sara A. Brenner, State University of New York (SUNY) Polytechnic Institute, College of Nanoscale Science, Nanobioscience Constellation, 257 Fuller Road, Albany, NY 12203
• Email:
  sbrenner@sunypoly.edu
• Federal Fiscal Year:
  2016
• Performing Organization:
  State University of New York at Albany
• Peer Reviewed:
  True
• Start Date:
  20120901
• Source Full Name:
  Journal of Visualized Experiments
• End Date:
  20150828
• Download URL:
  https://stacks.cdc.gov/view/cdc/202873/cdc_202873_DS1.pdf
• File Type:
  [PDF - 854.89 KB]
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7bfad28a152b0d417bfef86c30525ef5f45d0e31a721054c11bcc33f1d1c1e697efa849295ab0804ae10f263e3eaaa91b7623302afb6211e3c8b88324142d3ec