Hyperspectral imaging of nanoparticles in biological samples: simultaneous visualization and elemental identification

Details

• Personal Author:
  Brenner SA ; Frame MD ; Friedman AJ ; Gottipati A ; Neu Baker NM ; Peña Mdel P ; Tahiliani S
• Description:
  While engineered nanomaterials (ENMs) are increasingly incorporated into industrial processes and consumer products, the potential biological effects and health outcomes of exposure remain unknown. Novel advanced direct visualization techniques that require less time, cost, and resource investment than electron microscopy (EM) are needed for identifying and locating ENMs in biological samples. Hyperspectral imaging (HSI) combines spectrophotometry and imaging, using advanced optics and algorithms to capture a spectrum from 400 to 1000 nm at each pixel in an enhanced dark-field microscopic (EDFM) image. HSI-EDFM can be used to confirm the identity of the materials of interest in a sample and generate an image "mapping" their presence and location in a sample. Hyperspectral mapping is particularly important for biological samples, where ENM morphology is visually indistinct from surrounding tissue structures. While use of HSI (without mapping) is increasing, no studies to date have compared results from hyperspectral mapping with conventional methods. Thus, the objective of this study was to utilize EDFM-HSI to locate, identify, and map metal oxide ENMs in ex vivo histological porcine skin tissues, a toxicological model of cutaneous exposure, and compare findings with those of Raman spectroscopy (RS), energy-dispersive X-ray spectroscopy (EDS), and scanning electron microscopy (SEM). Results demonstrate that EDFM-HSI mapping is capable of locating and identifying ENMs in tissue, as confirmed by conventional methods. This study serves as initial confirmation of EDFM-HSI mapping as a novel and higher throughput technique for ENM identification in biological samples, and serves as the basis for further protocol development utilizing EDFM-HSI for semiquantitation of ENMs. [Description provided by NIOSH]
• Subjects:
  Histology Laboratories Microbiology Occupational Health Safety Toxicology
• Keywords:
  Analytical Instruments Analytical Processes Biological Materials Dispersion Imaging Techniques Laboratory Techniques Metal Oxides Microscopic Analysis Microscopy Nano-objects Nanomaterials Nanotechnology Optical Aids Optics Performance Capability Sampling Scanning Electron Microscopy Spectrophotofluorometry Spectroscopes Visual Aids Visual Images X-rays

  More +
• ISSN:
  1059-910X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  District of Columbia ; New York ; OSHA Region 2 ; OSHA Region 3
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  349-358
• Volume:
  79
• Issue:
  5
• NIOSHTIC Number:
  nn:20047930
• Citation:
  Microsc Res Tech 2016 May; 79(5):349-358
• Contact Point Address:
  Sara A. Brenner, MD, MPH, State University of New York (SUNY) Polytechnic Institute, College of Nanoscale Science, Nanobioscience Constellation, 257 Fuller Road, Albany, New York, 12203, United States of America
• Email:
  sbrenner@sunypoly.edu
• Federal Fiscal Year:
  2016
• Performing Organization:
  State University of New York at Albany
• Peer Reviewed:
  True
• Start Date:
  20120901
• Source Full Name:
  Microscopy Research and Technique
• End Date:
  20150828
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:18419c2189e3fff849b1b2faf2970b9a8a5755916f761116b834e1a70fe5a775e0f459ef82736fc117946ef8e4ad8b45bab92e91a7b049dd07986a8a0eeb5fe4
• Download URL:
  https://stacks.cdc.gov/view/cdc/202863/cdc_202863_DS1.pdf
• File Type:
  [PDF - 933.97 KB ]