Biodistribution of Inhaled Metal Oxide Nanoparticles Mimicking Occupational Exposure: A Preliminary Investigation Using Enhanced Darkfield Microscopy

Details

• Personal Author:
  Bezerra L ; Brenner SA ; Del Pilar Sosa Idelchik M ; Elder A ; Guttenberg M ; Neu-Baker NM ; Oberdörster G
• Description:
  Inhalation exposure to engineered nanomaterials (ENMs) may result in adverse pulmonary and/or systemic health effects. In this study, enhanced darkfield microscopy (EDFM) was used as a novel approach to visualizing industrial metal oxide nanoparticles (NPs) (silica, ceria, or alumina) in multiple tissue types following inhalation in rats mimicking occupational exposures. Advantages of EDFM over electron microscopy (EM) include reduced cost, time, and ease of sample preparation and operation. Following 4-6 hour inhalation exposures at three concentrations (3.5-34.0 mg/m3), lungs and secondary organs were harvested at 24 hours or 7 days post-exposure and prepared for brightfield (BF) microscopy and EDFM. NPs were visualized within the lung and associated lymphatic tissues and in major organs of excretion (liver, spleen, kidney). EDFM also revealed NPs within pulmonary blood vessels and localization within specific regions of toxicological relevance in liver and kidney, indicating pathways of excretion. Results demonstrate the utility of EDFM for rapid direct visualization of NPs in various tissue types and suggest the potential for metal oxide NPs to distribute to secondary tissues following inhalation exposure. Confirmation of the composition, distribution, and relative abundance of inhaled NPs will be pursued by combining EDFM with hyperspectral imaging (HSI) and mapping. [Description provided by NIOSH]
• Subjects:
  Blood Histology Laboratories Lung Metals Microbiology Occupational Health Respiratory System Safety Silicon Dioxide
• Keywords:
  Alumina Analytical Instruments Author Keywords: Inhalation Exposure Blood Vessels Cerium Dioxide Engineered Nanomaterials Enhanced Darkfield Microscopy Exposure Assessment Histological Samples Imaging Techniques Inhalation Inhalation Studies Kidneys Laboratory Animals Liver Lung Tissue Lymphatic System Metal-oxides Microscopic Analysis Microscopy Nanomaterials Nanoparticles Nanotechnology Organs Oxides Pulmonary Effects Pulmonary System Silica Splenic Tissue Toxic Effects Visual Images

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• ISSN:
  1864-0648
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  9
• Issue:
  10
• NIOSHTIC Number:
  nn:20050455
• Citation:
  J Biophotonics 2016 Oct; 9(10):987-993
• Contact Point Address:
  Sara A. Brenner, MD, MPH, State University of New York (SUNY) Polytechnic Institute, College of Nanoscale Science, 257 Fuller Road, Albany, New York 12203, United States
• Email:
  sbrenner@sunypoly.edu
• CAS Registry Number:
  Alumina (CAS RN 1344-28-1) ; Ceria (CAS RN 1306-38-3) ; Silica (CAS RN 7631-86-9)
• Federal Fiscal Year:
  2017
• Performing Organization:
  State University of New York at Albany
• Peer Reviewed:
  False
• Start Date:
  20120901
• Source Full Name:
  Journal of Biophotonics
• End Date:
  20150828
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fd04d4b2a5074733175320c8da823283f59a4c43f097828c41d2b9d740223bdb5d4bb5c1a85b4d773b28c8a448846c751100e09dfba5a62706276aa802d4efd7
• Download URL:
  https://stacks.cdc.gov/view/cdc/210784/cdc_210784_DS1.pdf
• File Type:
  [PDF - 548.37 KB ]