Apoptosis Induction In Vitro by Quartz and Kaolin Dusts

Details

• Personal Author:
  Gao N ; Keane M ; Wallace W
• Description:
  Respirable quartz and kaolin dusts were used to study induction of apoptosis in NR 8383 rat alveolar macrophages in vitro and in vivo by intratracheal instillation in male Sprague- Dawley rats. While quartz is a fibrogenic respirable mineral dust, kaolin, a silicate mineral similar to silica, is not generally considered fibrogenic, but has similar cytotoxic properties in vitro. Both dusts were <5 um fractions. In vitro concentrations ranged from 50-400 ug/ml, and the instilled animals received 4 or 20 mg in 0.5 ml saline. In vitro apoptosis methods included ELISA assays for nucleosomes, terminal deoxynucleotidyl transferase-mediated dUTP-fluorescein nick-end labeling (TUNEL) assays, and DNA ladders for apoptotic fragments. In vivo methods include TUNEL assays for lung tissue and flow cytometry for cells in bronchial alveolar lavage (BAL). In vitro results indicated that quartz induces apoptosis in a dose-dependent and time-dependent manner, while kaolin showed activity only a the highest (400 ug/ml) concentration in the TUNEL assay and slight activity at the 200 and 400 ug/ml points in the EUSA assay. Time course studies in vitro at 100 ug/rnl showed apoptosis at 6h, 12h, and 1,3, and 5 days for quartz, but only at 5 days for kaolin. The effect of serum on the dose-response is discussed. In vivo results show a time-dependent increase of apoptosis in the EUSA assay at both 4 and 20 mg doses, at times ranging from 2 days to 90 days. Kaolin does not show a significant response at any time points for the ELISA. Apoptotic cells as determined using flow cytometry showed a time-and concentration-dependent increase for quartz and no significant response for kaolin at times ranging from 2 to 90 days. [Description provided by NIOSH]
• Subjects:
  Animals Dust Irritants Laboratories Occupational Health Quartz Respiratory Tract Diseases Safety Silicon Dioxide
• Keywords:
  Animal-studies Cytotoxins In-vitro-study In-vivo-study Laboratory-animals Quartz-dust Respirable-dust Respiratory-irritants Respiratory-system-disorders
• ISSN:
  0025-7818
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  93
• NIOSHTIC Number:
  nn:20023115
• Citation:
  Med Lav 2002 Oct; 93(Suppl):S40
• CAS Registry Number:
  Kaolin (CAS RN 1332-58-7) ; Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  2003
• NORA Priority Area:
  Work Environment And Workforce: Mixed Exposures ; Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  False
• Source Full Name:
  La Medicina del Lavoro. 3rd International Symposium on Silica, Silicosis, Cancer and Other Diseases, S. Margherita Ligure, 21-25 October 2002
• Supplement:
  Suppl
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0193d239a770c405df2d658aaf728b1dc2ee1b2ce29fba704969eb3b9fc14c95aca3bd182f5187bfa17550233f29b0a36d8c002f233566129832d68906e05f9f
• Download URL:
  https://stacks.cdc.gov/view/cdc/199683/cdc_199683_DS1.pdf
• File Type:
  [PDF - 1.05 MB ]