Characterization of Alveolar Macrophage Eicosanoid

Details

• Personal Author:
  Demers LM ; Griffith JW ; Kuhn DC ; Riling S ; Stauffer JL
• Description:
  The relative activation of eicosanoid production which results from the exposure of the alveolar macrophage (AM) to mineral dusts is thought to be a key factor in the pathophysiology of occupational lung disease. We compared in vitro basal and silica-stimulated production of prostaglandin E2 (PGE2) and thromboxane A2 (TXA2) by AM from normal humans and non-human primates (Macaca nemestrina). In addition, we instilled mineral dusts directly into one lung of the non-human primate and evaluated AM eicosanoid production at two week intervals following dust instillation. Unstimulated AM from humans produce more PGE2 and TXA2 than do AM from M. nemestrina. However, in vitro exposure of AM from both species to silica dust produced a qualitatively similar increase in TXA2 production accompanied by no change in PGE2 production. Sequential analysis of AM eicosanoid production following a single bolus exposure to bituminous or anthracite coal dusts, titanium dioxide (TiO2) dust or crystalline silica showed marked variability among individual non-human primates in qualitative and quantitative aspects of dust-induced eicosanoid production. However, the rank order of potency of the different dusts (silica > anthracite > bituminous) correlated with epidemiological evidence relating the type of dust mined to the incidence of pneumoconiosis. These studies suggest that the non-human primate may serve as a model for the study of both the role of eicosanoids in the etiology of dust-induced occupational lung disease and the biochemical basis for individual variability in the response of lung cells to mineral dust exposure. [Description provided by NIOSH]
• Subjects:
  Animals Coal Dust Laboratories Occupational Health Quartz Respiratory Tract Diseases Safety Silicon Dioxide
• Keywords:
  Animal-studies Coal-dust Laboratory-animals Mineral-dusts Quartz-dust Respiratory-system-disorders Silica-dusts
• Series:
  Mining Publications
• ISSN:
  0090-6980
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  207-220
• Volume:
  46
• Issue:
  3
• NIOSHTIC Number:
  nn:10004809
• Citation:
  Prostaglandins 1993 Sep; 46(3):207-220
• CAS Registry Number:
  Quartz (SiO2) (CAS RN 14808-60-7) ; Silica (CAS RN 7631-86-9) ; TXB2 (CAS RN 54397-85-2)
• Federal Fiscal Year:
  1993
• Peer Reviewed:
  True
• Source Full Name:
  Prostaglandins
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ec52b651fac3b97a442cd5e78e2a6d97b056f1402bbcd0478b9b9bfedc3e0779285e1c1998ef42a5b1c5f29adacc7091495f2671588e88e891c674f6b1568efb
• Download URL:
  https://stacks.cdc.gov/view/cdc/219598/cdc_219598_DS1.pdf
• File Type:
  [PDF - 682.57 KB ]