Application of a Human Skin Tissue Culture Model in Dermal Absorption Studies of 3,3′, 4,4′-Tetrachlorobiphenyl-(TCB)

Details

• Personal Author:
  Qiao GL ; Riviere JE
• Description:
  TCB, one of the dioxin-like PCBs. demands much research and regulatory attention. To evaluate an in vitro generated human skin tissue culture model, cutaneous disposition of TCB under different exposure scenarios was investigated. Occlusive or non-occlusive doses of 14C- TCB were applied at 4 or 40 ug/cm2 in different vehicles including acetone, methylene chloride, a wateracetone mixture, and a soil-based mixture in flow-through diffusion cell studies (n=6-7/exposure condition). Significant exposure-dependent dermal absorption and disposition were observed. In vitro 8-hr absorption varied from 0.04% to 1.46% depending on vehicle dosage and occlusion. Much more TCB was absorbed into perfusate from soil than from liquid (organic or waterorganic mixture) vehicles although the total penetration amount was less. Surprisingly, TCB dermal absorption/penetration ratios, which can reflect dermal absorption efficiency, were decreased by occlusion (soil dos, 0.45->0.13) or by adding water to the acetone vehicle (0.06->0.02). A lower (1/10) TCB dose in soil or in acetone showed a 3-5X higher fractional dose absorption, but a lower (1/3-1/2) transdermal flux (ug/cm2/hr), than the higher dose in each vehicle. In conclusion, this human skin tissue culture model showed similar dermal absorption and disposition characteristics for TCB when compared to an in vitro porcine skin model. Dermal absorption data from liquid TCB doses might underestimate the risk of TCB non contaminated soil. Such observed exposure-dependent dermal disposition profiles need to be considered while assessing TCB dermal risk. [Description provided by NIOSH]
• Subjects:
  Automation Diagnosis Electrical Equipment And Supplies Electric Injuries Electricity Manufacturing And Industrial Facilities Mining Occupational Health Risk Assessment Safety
• Keywords:
  Absorption Rates Biphenyls In Vitro Studies Models Polychlorinated Biphenyls Risk Analysis Risk Factors Skin Skin Absorption Tissue Culture
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  North Carolina ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  54
• Issue:
  1
• NIOSHTIC Number:
  nn:20021045
• Citation:
  Toxicologist 2000 Mar; 54(1):150
• CAS Registry Number:
  Acetone (CAS RN 67-64-1) ; Dichloromethane (CAS RN 75-09-2) ; PCB 77 (CAS RN 32598-13-3)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 39th Annual Meeting, March 19-23, 2000, Philadelphia, Pennsylvania
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9958941fe050f253d9c21f7edd0f5674ce818705e86395192e02513bcb43de47eb47a418901eb3c636a8b07f156bc596a9cc4532f3e5dece0b7d3e4d9e841a1e
• Download URL:
  https://stacks.cdc.gov/view/cdc/198619/cdc_198619_DS1.pdf
• File Type:
  [PDF - 274.21 KB ]