Ovalbumin Aeroallergen Exposure-Response in Brown Norway Rats

Details

• Personal Author:
  Al-Humadi NH ; Fedan, Jeffery S. ; Hubbs, Ann F. ; Millecchia LL ; Nelson ER ; Robinson VA ; Siegel, Paul D.
• Description:
  A major route of exposure to allergens is through the respiratory tract. Comparatively few animal studies have used aerosolized high-molecular-weight allergens for sensitization, and in these studies, proper characterization of the aeroallergen exposure was usually missing. The purpose of this study was to profile the exposure-response relationship in Brown Norway rats (BNR) to well-characterized ovalbumin (OVA) aerosols. Rats were exposed 30 min/wk x 6 wk to respirable OVA aerosols from <1 mg/m(3) to 64 mg/m(3) air. Ovalbumin-specific circulating immunoglobulin (Ig)E, IgG, and IgA were measured throughout the study period. Rats were sacrificed 1 day after the last exposure. Pulmonary tissue was processed for histopathological and histochemical analysis. Tracheas were isolated, perfused, and assessed for in vitro responsiveness to methacholine. Serum concentrations of OVA-specific antibodies increased with both exposure concentration and number of exposures. The number of BNR with measurable titers also increased with both dose and time. Pulmonary inflammatory changes were noted only in BNR exposed to higher OVA concentrations (15 and 64 mg/m(3) air). Increased tracheal reactivity to methacholine was not found in any of the sensitized BNR. In summary, sustained aeroallergen concentration-dependent changes in specific antibody responses and pulmonary inflammation have been demonstrated. [Description provided by NIOSH]
• Subjects:
  Aerosols Animals Antibodies Environmental Monitoring Hypersensitivity Laboratories Occupational Health Respiratory Tract Diseases Safety
• Keywords:
  Allergens Animal-studies Antibody-response Exposure-assessment Exposure-levels Histochemical-analysis Histopathology Laboratory-animals Pulmonary-system-disorders Respiratory-system-disorders Sensitization

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• ISSN:
  0895-8378
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  245-261
• Volume:
  12
• Issue:
  3
• NIOSHTIC Number:
  nn:20020810
• Citation:
  Inhal Toxicol 2000 Mar; 12(3):245-261
• Contact Point Address:
  Paul D. Siegel, PhD, Team Leader / Bioorganic Chemistry, Analytical Service Branch, HELD/NIOSH, 1095 Willowdale Road, Morgantown, WV 26505, USA
• Email:
  pds3@cdc.gov
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  Inhalation Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0264f409c31b1026722a26a7f94e5de9d5a978ceffa61753c0906b7e0f1dc956cd312a38cdb22c02f5ab5019bad6586a13f6f44d09cc62ddca9e8e781768aab2
• Download URL:
  https://stacks.cdc.gov/view/cdc/198475/cdc_198475_DS1.pdf
• File Type:
  [PDF - 418.07 KB ]