Immunomodulation of the IGE Response to Natural Rubber Latex (NRL) Proteins by Endotoxin and Glutaraldehyde

Details

• Personal Author:
  Howell MD ; Johnson CM ; Leakakos T ; Meade BJ ; Tomazic-Jezic V ; Truscott W
• Description:
  Reduction of the protein and powder content of NRL gloves has been recommended to reduce the incidence of latex sensitization and symptoms associated with NRL exposure. A better understanding of the effects of co-exposure with other irritant/sensitizers on the development of NRL allergy will be helpful in developing effective intervention strategies. These studies were conducted to investigate the effect of co-exposure to the common health care contaminants endotoxin and glutaraldehyde on the development of an IgE response to NRL. Endotoxin was tested at 50 -25,000 EU while concentrations of glutaraldehyde tested were representative of the concentrations found in cold sterilant solutions (0.1 - 1 %) and aerosol levels surrounding the permissible exposure limits (PEL, 0.05 - 1 ppm). Female BALB/c mice were concurrently exposed to endotoxin (intranasally, subcutaneously) or glutaraldehyde (dermally) and 25 microg of NRL proteins 5 days a week for up to 17 weeks. Mice were tail bled prior to exposure and bi-weekly thereafter to obtain total and specific serum immunoglobulin levels. Animals exposed concurrently to endotoxin and NRL exhibited a dose dependent decrease in total and NRL specific serum IgE levels reaching >50% suppression and increase in serum IgG1 and IgG2a levels. Mice exposed to glutaraldehyde in concentrations consistent with cold sterilant solutions exhibited decreases in total (>50%) or NRL specific IgE levels when compared to mice exposed to NRL alone. However, upon co-exposure to levels of glutaraldehyde in the range of the PEL a dose responsive increase in both total (3 fold) and NRL specific (>40 fold) IgE was observed. These studies demonstrated that endotoxin induced an immunoglobulin class switch whereas glutaraldehyde augmented the IgE response to NRL proteins. [Description provided by NIOSH]
• Subjects:
  Aerosols Animals Endotoxins Environmental Monitoring Hypersensitivity Irritants Laboratories Occupational Health Safety
• Keywords:
  Allergic-reactions Animal-studies Exposure-assessment Exposure-limits Health-care Laboratory-animals Sensitization
• ISSN:
  1096-6080
• Publisher:
  Oxford University Press
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; District of Columbia ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  174-175
• Volume:
  60
• Issue:
  1
• NIOSHTIC Number:
  nn:20025780
• Citation:
  Toxicologist 2001 Mar; 60(1):174-175
• CAS Registry Number:
  Glutaraldehyde (CAS RN 111-30-8)
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 40th Annual Meeting, March 25-29, 2001, San Francisco, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c5111b031cd71f9839cf8e288c3250c90392c18a8a952f088fbe43ba3c4e7b07d0b7f574a0193296fd3c545917b0c654357b603a181d2026076c36f2c6fb8ccd
• Download URL:
  https://stacks.cdc.gov/view/cdc/197007/cdc_197007_DS1.pdf
• File Type:
  [PDF - 204.28 KB ]