Evaluation of IL-9, IL-10, IL-13 and IL-15 as Biomarkers of Dermal Exposure to Respiratory Sensitizers

Details

• Personal Author:
  Manetz TS ; Meade BJ ; Pettit D
• Description:
  Differential modulation of mRNA and protein levels for interleukin-4 (IL-4), IL10 and interferon gamma by respiratory and T cell mediated sensitizers has been demonstrated. The following investigation examined the mRNA expression patterns of multiple cytokines associated with respiratory sensitization for modulation using a Multiprobe Ribonuclease Protection Assay. Female BALB/c mice were dermally exposed on the shaven dorsal lumbar area with either vehicle or test article on Day 0 and Day 5 followed 5 days later by exposure on the ear pinna for 3 consecutive days to chemicals known to primarily induce irritation (Sodium 1auryl Sulfate), respiratory sensitization (Toluene Diisocyanate), or T cell-mediated hypersensitivity (Dinitrofluorobenzene) responses. Draining lymph nodes were excised and mRNA isolated immediately or following 24 or 48 hrs in culture with Concanavalin A (Con A). Differential expression of cytokine mRNA was most notable in animals receiving test article only on the ears (no previous induction) following 24 hr incubation with Con A. The response for IL-4, IL-10, and IFN-y was consistent with previous studies by others. In addition, IL-9, IL-13 and IL-15 were significantly elevated only following TDI exposure. The most robust modulation (>200 fold increase over control) was observed for IL-13 which shares many biological activities with IL-4 and has been shown to be required for the induction of airway hyper-responsiveness in mice. Further investigations of these cytokines may provide additional insight into the mechanisms of chemically induced respiratory sensitization and provide endpoints for screening methods aimed at identifying chemicals with the capacity to elicit pulmonary sensitization. [Description provided by NIOSH]
• Subjects:
  Animals Biological Factors Biomarkers Hypersensitivity Laboratories Occupational Health Respiratory Hypersensitivity Respiratory Tract Diseases Safety
• Keywords:
  Animal-studies Biological-factors Exposure-levels Laboratory-animals Pulmonary-system-disorders Respiratory-hypersensitivity Respiratory-system-disorders Sensitization
• ISSN:
  1096-6080
• Publisher:
  Oxford University Press
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Maryland ; OSHA Region 3 ; Virginia ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  OD - Office of the Director
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  8
• Volume:
  60
• Issue:
  1
• NIOSHTIC Number:
  nn:20025745
• Citation:
  Toxicologist 2001 Mar; 60(1):8
• CAS Registry Number:
  Sodium dodecyl sulfate (CAS RN 151-21-3)
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 40th Annual Meeting, March 25-29, 2001, San Francisco, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a2c1fb1ceee10e8868a93533505b0f2133ca85181da587756385019a5b251a4a2b4cc714b4050f95764e84c61de4948a034be6d2593245a12e5f9c0801fcab88
• Download URL:
  https://stacks.cdc.gov/view/cdc/196990/cdc_196990_DS1.pdf
• File Type:
  [PDF - 127.21 KB ]