Beryllium-Induced Gene Changes in a Mouse Alveolar Macrophage Cell Line

Details

• Personal Author:
  Flint MS ; Tinkle SS
• Description:
  Chronic Beryllium Disease (CBD) is an occupationally acquired lung disease that occurs as a cell mediated immune response to beryllium, resulting in the development of noncaseating granulomas. We hypothesized that the identification of early genes associated with beryllium exposure may give important insights into the role of macrophages in CBD. We investigated gene expression in a mouse alveolar macrophage cell line incubated in the presence of absence of 10microM BeSO4 for 4 hours. We utilized Affymetrix oligonucleotide arrays consisting of approximately 12000 genes to detect differential changes in gene expression in beryllium-treated cells compared to controls. We found that beryllium caused significant changes in the expression of numerous genes encoding; heat shock proteins, cytokines, adhesion molecules, cytokine receptors, signalling molecules and transcriptional activators and repressors. HSP70 and MHC class III region were elevated 10-fold in BeSO4 treated macrophages. However, IL- 1[beta], ICE, ICAM-1 and IL-10R were all decreased by beryllium. We verified beryllium-modulation of these genes by real time quantification (TaqmanTMPCR). Our studies identify beryllium-regulated genes and suggest that HSPs and cytokine genes play pivotal roles in an early immune response to beryllium. [Description provided by NIOSH]
• Subjects:
  Beryllium Genetics Immune System Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Beryllium-disease Genes Genetic-factors Immune-reaction Immune-system Immunology In-vitro-studies Lung-disease Lung-function Pulmonary-system-disorders Respiratory-system-disorders

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• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Maryland ; OSHA Region 3
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  17
• Issue:
  7
• NIOSHTIC Number:
  nn:20023201
• Citation:
  FASEB J 2003 Apr; 17(7):C98
• CAS Registry Number:
  Beryllium (CAS RN 7440-41-7)
• Federal Fiscal Year:
  2003
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal, Immunology 2003, Denver, Colorado May 6-10, 2003
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:55702ff27d5048a18a84472e5a3832f425990605b03b1be4b637e8462b2cb2047813257fdc68856013e2c250febf4cc5a3c143d55da8752fdec768a811347dee
• Download URL:
  https://stacks.cdc.gov/view/cdc/195755/cdc_195755_DS1.pdf
• File Type:
  [PDF - 620.91 KB ]