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Partial IL-10 Inhibition of the Cell-Mediated Immune Response in Chronic Beryllium Disease

Public Domain


Details

  • Personal Author:
  • Description:
    Chronic beryllium disease (CBD) provides a human disorder in which to study the delayed type IV hypersensitivity response to persistent Ag that leads to noncaseating pulmonary granuloma formation. We hypothesized that, in CBD, failure of IL-10 to modulate the beryllium-specific, cell-mediated immune response would result in persistent, maximal cytokine production and T lymphocyte proliferation, thus contributing to the development of granulomatous lung disease. To test this hypothesis, we used bronchoalveolar lavage cells from control and CBD subjects to evaluate the beryllium salt-specific production of endogenous IL-10 and the effects of exogenous human rIL-10 (rhIL-10) on HLA expression, on the production of IL-2, IFN-gamma, and TNF-alpha, and on T lymphocyte proliferation. Our data demonstrate that beryllium-stimulated bronchoalveolar lavage cells produce IL-10, and the neutralization of endogenous IL-10 does not increase significantly cytokine production, HLA expression, or T lymphocyte proliferation. Second, the addition of excess exogenous rhIL-10 partially inhibited the beryllium-stimulated production of IL-2, IFN-gamma, and TNF-alpha; however, we measured no change in T lymphocyte proliferation or in the percentage of alveolar macrophages expressing HLA-DP. Interestingly, beryllium salts interfered with an IL-10-stimulated decrease in the percentage of alveolar macrophages expressing HLA-DR. We conclude that, in the CBD-derived, beryllium-stimulated cell-mediated immune response, low levels of endogenous IL-10 have no appreciable effect; exogenous rhIL-10 has a limited effect on cytokine production and no effect on T lymphocyte proliferation or HLA expression. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    0022-1767
  • Document Type:
    Text
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Division:
    HELD - Health Effects Laboratory Division
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Volume:
    163
  • Issue:
    5
  • NIOSHTIC Number:
    nn:20025376
  • Citation:
    J Immunol 1999 Sep; 163(5):2747-2753
  • Contact Point Address:
    Dr. Sally S. Tinkle, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
  • Email:
    sft3@cdc.gov
  • CAS Registry Number:
    Silver (CAS RN 7440-22-4)
  • Federal Fiscal Year:
    1999
  • Peer Reviewed:
    True
  • Source Full Name:
    The Journal of Immunology
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:17f46176b6ec7769b44d0ac9891b0cbe26498e3eb7b200c453aab8bbc6083d696d0445d95f07a1c4d9df76887f09e69243e048599b1d31fa46cf0f2d8d8a94b1
  • Download URL:
  • File Type:
    Filetype[PDF - 322.54 KB ]
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