Silver nanowires induced inflammation in an in vitro human alveolar lung model

Details

• Personal Author:
  Estep CA ; Hussain SM ; Roberts, Jennifer R. ; Schaeublin NM
• Description:
  The goal of this study was to evaluate the biological response following inhalation exposure to silver nanowires (Ag-NWs). Nanomaterials have been shown to penetrate deep into the alveolar regions of the lung, so we used a human alveolar lung co-culture model previously established in our lab as our model for inhalation exposure. The Ag-NWs were synthesized by nanoComposix and were 4 and 20 mu m in length, with a similar diameter of approximately 90 nm. Changes in cellular morphology and the cellular interaction of the Ag-NWs with the cells was assessed using ultra-resolution microscopy after a 24 h exposure to 200 ng/ml of the Ag-NWs. The cell morphology and interaction of the Ag-NWs demonstrated that both lengths of Ag-NWs were interacting with the cells, but normal morphology indicated that they were not toxic. In addition, the co-cultures were exposed to Ag-NWs (concentrations ranging from 0-200 ng/ml) and cell viability was assessed using MTS to evaluate mitochondrial function. Our viability assays indicated that both Ag-NWs did not alter cell viability and that there was no toxicity. In addition, inflammatory responses were evaluated using ELISA assays to determine the secretion of 12 different cytokines. Of the cytokines evaluated, the Ag-NWs demonstrated an increase in secretion of several of them including IL-6, IL-8 and Interferon Gamma indicating that the Ag-NWs caused inflammation. Therefore, while the Ag-NWs were not toxic to the cells, they did cause irritation and an inflammatory response. [Description provided by NIOSH]
• Subjects:
  Cytology Irritants Laboratories Lung Lung Diseases Occupational Health Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Biological-effects Cell-biology Cell-morphology Laboratory-testing Lung-irritants Lung-tissue Molecular-biology Nanotechnology Pulmonary-disorders Quantitative-analysis Respiratory-hypersensitivity Respiratory-irritants Statistical-analysis Tissue-disorders

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  District of Columbia ; Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  120
• NIOSHTIC Number:
  nn:20038619
• Citation:
  Toxicologist 2011 Mar; 120(Suppl 2):468
• CAS Registry Number:
  Silver (CAS RN 7440-22-4)
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 50th Annual Meeting and ToxExpo, March 6-10, 2011, Washington, DC
• Supplement:
  2
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:61665087a0c7ecc24c12dcafad0d5c2ec32ccd219ccbb5056b94fcb2a7e9cccd0c26a7c28dc613c4ef7a1209019fdc16f971370d49b3c6cacfc14c6f0df15d2e
• Download URL:
  https://stacks.cdc.gov/view/cdc/192268/cdc_192268_DS1.pdf
• File Type:
  [PDF - 1.10 MB ]