SWCNT exposure of alveolar epithelial cells and macrophages induced OPN and TGF-beta1 response

Details

• Personal Author:
  Kisin E ; Murray AR ; Shvedova AA ; Waltz MJ
• Description:
  Exposure of the lung to carbonaceous nanoparticles: single walled carbon nanotubes (SWCNT) leads to the development of pulmonary fibrosis (PF) and induction of a network of pro-fibrotic/pro-inflammatory cytokines including TGF-beta1 and osteopontin (OPN). TGF-beta1 is involved in fibrotic remodeling including fibroblast differentiation and enhanced deposition of collagen in the extracellular matrix. As part of the inflammatory cascade, OPN acts as a chemoattractant to guide polymorphonuclear neutrophils (PMN) and macrophages (M Phi) to the area of insult/injury and is also involved in collagen deposition. Potential interactions between OPN and TGF-beta1 have not been investigated even though both cytokines have been shown to be upregulated in response to SWCNT. We hypothesize that OPN increase in response to SWCNT potentiate TGF-beta1 production in lung cells. To test our hypothesis two cell types, alveolar epithelial cells (MLE-15) and RAW 264.7 M Phi were treated with SWCNT (6 mu g/cm2 - 48 mu g/cm2, for 24 hours). Exposure of the cells to SWCNT resulted in significantly enhanced OPN released found in the incubation medium (39.1% for MLE-15 and 38.6% for RAW 264.7 M Phi vs. respective controls). Using an OPN antibody we demonstrated an inhibition of OPN production in response to SWCNT. Further studies are needed to evaluate interplay between OPN and TGF-beta1 and their role in SWCNT induced pulmonary fibrosis. [Description provided by NIOSH]
• Subjects:
  Cytotoxins Hazardous Substances Irritants Lung Lung Diseases Occupational Health Particulate Matter Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Biological-effects Cell-biology Cell-damage Cellular-reactions Cytotoxic-effects Fibrogenicity Fibrous-bodies Immunotoxins Lung-disorders Lung-fibrosis Lung-irritants Lung-tissue Molecular-biology Nanotechnology Particulates Pulmonary-disorders Pulmonary-system-disorders Quantitative-analysis Respiratory-hypersensitivity Respiratory-irritants Respiratory-system-disorders Statistical-analysis Tissue-disorders Toxic-effects Toxic-materials

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  District of Columbia ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  120
• NIOSHTIC Number:
  nn:20038618
• Citation:
  Toxicologist 2011 Mar; 120(Suppl 2):463
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 50th Annual Meeting and ToxExpo, March 6-10, 2011, Washington, DC
• Supplement:
  2
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4ac337418b5151b7c7362fb84a6ff767b6ae4f374187b29a895613d5661b02a43b179cc86e5de6b08566caec7ff3d7bfdc736d821c1533c8aa23e1dcb344a346
• Download URL:
  https://stacks.cdc.gov/view/cdc/192267/cdc_192267_DS1.pdf
• File Type:
  [PDF - 1.10 MB ]